作物学报2017，Vol.43Issue(10)：1426-1433,8.DOI:10.3724/SP.J.1006.2017.01426
一个水稻温敏黄化突变体的表型分析和基因定位
Phenotypes and Gene Mapping of a Thermo-sensitive Yellow Leaf Mutant of Rice
摘要
Abstract
Phenotypic analysis and gene mapping of rice leaf color could lay a foundation for map-based cloning of related genes and the function research of rice photosynthetic system. Leaf yellow mutant dy1 was obtained from rice cultivar "Nanjing 11"(abbreviated as NJ11) mutated by ethyl methanesulfonate (EMS). The mutant dy1 showed leaf yellowing at seedling stage and maturity stage in natural environment, with an abnormal structure of thylakoids under TEM, and significant differences in plant height, tiller number, seed setting rate and so on. The mutant dy1 showed albinism in 20℃, etiolation in 25℃ and virescence in 30℃. The rice leaf yellow mutant dy1 was controlled by a single recessive gene. F2 population was constructed by crossing the mutant with "02428", and the mutant phenotypes of extreme individuals were selected to map gene dy1. The gene was located in the 115 kb region of the long arm of chromosome 1, and contained 16 ORFs. Sequencing analysis showed that LOC_Os01g73450 controlling a uracil nucleotide kinase, with a single base substitution in the junction of the fourth intron and the fifth exon in dy1, might be a candidate gene. And the expression of genes related to chloroplast synthesis was significantly decreased, indicating dy1 may involve in the chloroplast synthesis关键词
水稻/温度敏感型/遗传分析/叶色/基因定位Key words
Rice/Thermo-sensitive/Genetic analysis/Leaf color/Gene mapping引用本文复制引用
张天雨,周春雷,刘喜,孙爱伶,曹鹏辉,Thanhliem NGUYEN,田云录,翟虎渠,江玲..一个水稻温敏黄化突变体的表型分析和基因定位[J].作物学报,2017,43(10):1426-1433,8.基金项目
本研究由国家重点研发计划项目(2016YFD0100101-08),江苏省科技支撑计划项目(BE2015363),江苏省农业科技自主创新项目[CX(16)1029]和安徽省科技重大专项(16030701068)资助.This study was supported by the National Key Research and Development Program of China (2016YFD0100101-08),the Science and Tech-nology Support Project of Jiangsu Province (BE2015363),the Independent Innovation Project for Agricultural Science and Technology of Jiangsu Province [CX(16)1029],and the Science and Technology Major Projects of Anhui Province (16030701068). （2016YFD0100101-08）
