作物学报2017，Vol.43Issue(10)：1458-1467,10.DOI:10.3724/SP.J.1006.2017.01458
大豆转录因子GmMYB52的克隆、表达及结合功能分析
Isolation, Expression and Binding Function Analysis of the Transcription Fac-tor GmMYB52 in Soybean
摘要
Abstract
The MYB type transcription factors are involved in plant development and response to abiotic stress. GmMYB52 was significantly up-regulated after salt treatment. In order to gain more information about GmMYB52, GmMYB52 of Williams 82 was cloned by RT-PCR. Bioinformatic analysis showed the CDS of GmMYB52 was 1083 bp, encoding 360 amino acid residues. A MYB domain was found in the region of 110 to 160 amino acid residues from N-terminal. Blast results showed that GmMYB52 are highly homologous to GmMYB62, AtMYBSt1 from Arabidopsis, MtMYB52 from Medicago sativa, OsMYBS3 from Oryza sativa, and CcMYB-like protein J from Cajanus cajan. Quantitative PCR (qPCR) results indicated that the transcription level of GmMYB52 was upregulated under ABA and low temperature stresses, under salt, drought and cold stresses, show a bimodal pattern. GmMYB52 was nearly expressed in all detected tissues, except in pods at maturing stage, and its expression level was relatively higher at seedling or flowering stages than those at maturing stage. The transcription level of GmMYB52 was high in stem, leaf, and flower, during seedling and blooming stages, and low in root and pods during maturity stage. Subcellular localization results showed that GmMYB52 was located in the nucleus which is in agreement with the localization characteristics of typical transcription factors. Yeast hybrid assay indicated that GmMYB52 had transcriptional activation functions and could bind to several MYB cis-acting element motifs. In conclusion GmMYB52 is a typical 1R-MYB transcription factor, and able to bind MYB cis-acting ele-ment motifs. We speculate GmMYB52 is involved in response to the abiotic stress and ABA signal transduction pathway.关键词
大豆/GmMYB52/表达分析/转录激活功能/结合功能Key words
Soybean/GmMYB52/Expression analysis/MYB motif/Transcriptional activation activity引用本文复制引用
许玲,王元琮,何晓兰,黄益洪,徐照龙,邵宏波,张大勇..大豆转录因子GmMYB52的克隆、表达及结合功能分析[J].作物学报,2017,43(10):1458-1467,10.基金项目
本研究由国家自然科学基金项目(31600211,31101166),江苏省自然科学基金项目(BK20151364)和江苏省农业科技自主创新项目(CX(15)1005)资助. This study was supported by the National Natural Science Foundation of China (31600211,31101166),the Natural Science Foundation of Jiangsu Province,and the Independent Innovation Project for Agricultural Science and Technology of Jiangsu Province [CX(15)1005]. （31600211,31101166）
