作物学报2017，Vol.43Issue(10)：1499-1506,8.DOI:10.3724/SP.J.1006.2017.01499
新型香稻渝恢2103香味分子遗传特性分析
Molecular Genetic Characteristics of Fragrance in a New Fragrant Rice Vari-ety Yuhui 2103
摘要
Abstract
A major component 2-acetyl-1-pyrroline (2AP) in fragrance is one of the important indices of high-quality rice, how-ever, the biosynthetic pathway of 2AP has not been demonstrated clearly. The betaine aldehyde dehydrogenase 2 (BADH2) is considered to be closely related to 2AP biosynthesis. In this study, three mutations in Badh2 gene were screened from thirty rice varieties and a new fragrant rice variety Yuhui 2103 was discovered. The new variety has no mutation in the coding region of Badh2 gene and the Badh2 allele of Yuhui 2103 can complement the defect of the badh2-E7 allele in Yixiang 1B. From crosses between Yuhui 2103 and non-fragrant varieties the segregating ratios of F2 fragrant to non-fragrant individuals were 9:7, while from crosses between Yuhui 2103 and fragrant varieties the segregating ratios of F2 fragrant to non-fragrant individuals were 7:9, indicating that fragrance of Yuhui 2103 is not controlled by only one gene. Furthermore, expression patterns of genes involved in 2AP biosynthesis were examined by the quantitative real-time PCR (qRT-PCR) in Nipponbare, Yuhui 2103, and Yixiang 1B. There was no significant difference in transcription level of Badh2 in Nipponbare and Yuhui 2103, however, the transcription levelof Badh2 in Yixiang 1B was unexpectedly high. The expression levels of most of the genes involved in proline and glutamatic acid metabolism were significantly higher in Yixiang 1B than that in Nipponbare and Yuhui 2103. It is proposed that 2AP biosyn-thesis in Yixiang 1B is both influenced by Badh2 and the genes involved in proline and glutamatic acid metabolism, however, there is no necessary relationship between these genes and the fragrance of Yuhui 2103. The novel genetic characters of Yuhui 2103 may bring new breakthrough to the study of fragrance formation mechanism in rice.关键词
香稻/2-乙酰-1-吡咯啉/脯氨酸/谷氨酸/Badh2/遗传特性Key words
Fragrant rice/2-Acetyl-1-pyrroline/Proline/Glutamatic acid/Badh2/Genetic characteristics引用本文复制引用
王春萍,唐荣莉,李经勇,雷开荣,张现伟,白文钦,蒋晓英,吴红,林清,唐永群,姚雄,张巫军..新型香稻渝恢2103香味分子遗传特性分析[J].作物学报,2017,43(10):1499-1506,8.基金项目
本研究由重庆市农发良种创新暨重大科研推广项目(NKY2016AA003),重庆市农发基础科研项目(NKY2016AC024),重庆市基础与前沿研究计划项目(cstc2016jcyjA0091)和重庆市基本科研业务费(2014cstc-jbky-00549)资助. This study was supported by Chongqing Agriculture Seed Innovation and Major Research Extension Project (NKY2016AA003),Chongqing Agriculture Basic Research Project (NKY2016AC024),Chongqing Basic and Frontier Research Program (cstc2016jcyjA0091),and Chongqing Basic Research Fund (2014cstc-jbky-00549). （NKY2016AA003）
