中国现代中药2017，Vol.19Issue(7)：917-923,959,8.DOI:10.13313/j.issn.1673-4890.2017.7.004
基于DNA条形码技术鉴别5种列当属药用植物
Study on Identification of Five Species of Genus Orobanche with DNA Barcoding Technology
摘要
Abstract
Objective:To establish a rapid,accurate and standardized method for identifying five species from genus Orobanche with DNA barcoding technology.Methods:Total DNA of 5 kinds of medicinal plants from genus Orobanche were extracted.ITS,rbcL,trnL-F and matK gene sequence amplified by PCR.PCR product was directly sequenced,and the results were aligned and analyzed with different software (CONTIG、BioEdit、MEGA6.0) to construct the phylogeny trees.Results:The 200 complete ITS,rbcL,trnL-F and matK sequences of 5 species from genus Orobanche have been obtained,and the length of 4 sequences region varied from 364 to 1240 bp.They were distinguished by trnL-F,ITS or matK sequence.Conclusion:RbcL sequence was not suitable to identify 5 species from the genus Orobanche,but ITS and trnL-F region could be a pretty good marker to distinguish these species,and it will be a potential molecular identification method for identifying Orobanche plants.关键词
分子鉴别/DNA条形码/列当属Key words
Molecular identification/DNA barcoding/Orobanche L.引用本文复制引用
韩奇亨,汤健,王晓琴,李旻辉..基于DNA条形码技术鉴别5种列当属药用植物[J].中国现代中药,2017,19(7):917-923,959,8.基金项目
国家自然科学基金项目(81260615) （81260615）
