中国现代中药2017，Vol.19Issue(7)：934-938,5.DOI:10.13313/j.issn.1673-4890.2017.7.007
基于“形态+分子”方法对疑似灵芝属样品的基原探究
A Primary Study on Classification and Identification of Two Samples Based on Morphological Characteristics and Molecular Analyses
摘要
Abstract
Objective:In order to identify and classify two samples which were suspected as Ganoderma,the morphological characteristics and molecular analyses were performed.Methods:Basing on primary identification of them,the rDNA-ITS region were amplified thereby,the rDNA-ITS amplicon was characterized by cloning,sequencing,blasting in GenBank and phylogenetic analyses using MEGA.Results:The rDNA-ITS entire sequences of the two species was 752 bp and 753 bp,respectively.The sequences both related to the Pyrrhoderma adamantinum were available in GenBank,and the similarity reached 99.0％.The cluster analysis suggested that the relationship between the two samples and P.adamantinum was closer,however,the relationship between the two samples and the G anoderma were relatively farther.The morphological and microscopic characteristics combined with the sequence analysis demonstrated that the two samples are P.adamantinum.Conclusion:The molecular biology methods of rDNA-ITS region analysis provides a technical support and theoretical reference for accurate identification on suspected fungi samples.关键词
分子鉴定/极硬红皮孔菌/ITS序列Key words
Molecular identification/Pyrrhoderma adamantinum/ITS引用本文复制引用
董晓曼,王亚君,查良平,蒋超,金艳,赵玉洋,袁媛..基于“形态+分子”方法对疑似灵芝属样品的基原探究[J].中国现代中药,2017,19(7):934-938,5.基金项目
中医药行业科研专项(201407003) （201407003）
