中国现代中药2017，Vol.19Issue(7)：939-943,964,6.DOI:10.13313/j.issn.1673-4890.2017.7.008
基于ITS2序列的朱砂根及其混伪品分子鉴定
Molecular Identification of Ardisiae Crenatae Radix and Its Adulterants Based on ITS2 Sequence
摘要
Abstract
Objective:This research aims to identify Ardisiae Crenatae Radix and its adulterants accurately using the ITS2 sequence.It will provide the foundation for its clinical medication and accurate market standardized management.Methods:Ardisia Crenatae Radix and its adulterants were identified and distinguished by ITS2 barcode,one marker of DNA barcoding.Genomic DNA of Ardisia crenata and its adulterants were extracted and then the ITS2 regions were obtained by PCR amplification and sequenced bidirectionally.The ITS2 sequences were aligned and the genetic distances were computed using MEGA 6.0 in accordance with the Kimura 2-parameter (K2P) model.Results:The length of A.crenata sequence is 217 bp and the average G + C content was 59.1％.There were ten variable sites of the ITS2 regions ofA.crenata and 48 ITS2 sequences of A.crenata included nine haplotypes.Except for A.crenata var.bicolor,the maximum intra-specific K2P genetic distance of Ardisiae Crenatae Radix and its adulterants was less than the minimum inter-specific K2P genetic distance.Conclusion:The experimental results indicated that the ITS2 sequence could be a good marker to identify Ardisia Crenatae Radix and its adulterants.But the relationship of A.crenata and A.crenata var.bicolor need to be further studied.关键词
朱砂根/鉴别/ITS2序列/DNA条形码/混伪品Key words
Ardisiae Crenatae Radix/identification/ITS2 sequence/DNA barcoding/adulterants引用本文复制引用
陈新连,周建国,马双姣,姚辉,林余霖,王瑀..基于ITS2序列的朱砂根及其混伪品分子鉴定[J].中国现代中药,2017,19(7):939-943,964,6.基金项目
国家科技支撑计划(2011BAI07B08) （2011BAI07B08）
中国医学科学院医学与健康科技创新工程(2016-I2M-3-016) （2016-I2M-3-016）
