癌变·畸变·突变2017，Vol.29Issue(5)：335-339,5.DOI:10.3969/j.issn.1004-616x.2017.05.003
组蛋白去乙酰化酶抑制剂联合紫杉醇抑制宫颈癌细胞增殖的体外实验
Histone deacetylase inhibitor restrained proliferation of human cervical cancer cells
摘要
Abstract
OBJECTIVE: To investigate the inhibitory effect of paclitaxel and SAHA on human cervical cancer cells. METHODS:Human cervical cancer (HeLa) cells were treated with paclitaxel (10 nmol/L),SAHA (10 μmol/L), or paclitaxel (10 nmol/L) + SAHA (10 μmol/L) for 24 hours or 48 hours. The inhibitory rates of HeLa cells were determined by MTT assay. p27 mRNA levels were determined by RT-PCR assay,protein levels of Ac-H4 were identified by Western blot and IC of paclitaxel was calculated by SPSS 16.0. RESULTS:Results from the MTT assay show that the inhibition50 rates from exposure to the combination of paclitaxel and SAHA were significantly higher than those from the single treatment groups (P<0.01). Similarly,RT-PCR assay show that the p27 mRNA levels from the combined treatments were significantly higher than from single treatments (P<0.01). From combined treatments,the inhibitory rates were 54.27％± 4.02％ with 24 h treatment and 77.02％±3.86％ with 48 h treatment,the mRNA level was 10.601±0.673,the proteinlevel of Ac-H4 was 1.282±0.033,and IC was reduced significantly. CONCLUSION:SAHA in combination with50 paclitaxel significantly inhibited HeLa cell proliferation by enhancing the level of histone acetylation and inducing the expression of tumor suppressor genes in vitro.关键词
SAHA/紫杉醇/宫颈癌/HeLa细胞/细胞增殖Key words
SAHA/paclitaxel/cervical cancers/HeLa cell/cell proliferation分类
医药卫生引用本文复制引用
刘好,赵滢滢,金平,许改霞,郭宇然,张可,王晓梅,张蕾..组蛋白去乙酰化酶抑制剂联合紫杉醇抑制宫颈癌细胞增殖的体外实验[J].癌变·畸变·突变,2017,29(5):335-339,5.基金项目
深圳市科技创新基础研究项目(JCYJ20140415163543959,JCYJ 20140418091413563) （JCYJ20140415163543959,JCYJ 20140418091413563）
