癌变·畸变·突变2017，Vol.29Issue(5)：340-345,351,7.DOI:10.3969/j.issn.1004-616x.2017.05.004
甲醛对人肝癌细胞株HepG2内质网应激相关Bip和CANX mRNA及蛋白表达水平的影响
Effects of formaldehyde on expression of endoplasmic reticulum stress-related Bip and CANX mRNA and proteins of HepG2 cell line
摘要
Abstract
OBJECTIVE: To investigate whether formaldehyde can induce endoplasmic reticulum stress in hepatocytes. METHODS:Hoechst 33258 was used for nuclear staining to observe morphological changes of hepatocytes in different formaldehyde concentration groups. The human hepatocellular carcinoma cell line HepG2 was treated with formaldehyde (FA) solution at 0.004 mmol/L,0.02 mmol/L and 0.1 mmol/L for 24 h and 48 h respectively. Expression of Bip and CANX mRNA in stress-related genes was detected by fluorescence quantitative PCR (qPCR). Western blot was used to detect the expression of stress-related immunoglobulin heavy chain binding protein (Bip) and connexin (CANX) in endoplasmic reticulum. RESULTS:Cells in the negative control group and the low dose group were significantly higher with Hoechst 33258 staining than in the control group (P<0.05). Results of qPCR show that expression of Bip and CANX mRNA in each dose group was not significantly different from that in the negative control group. Results of Western blot show that there was no significant difference in the expression of Bip and CANX proteins among the different doses of FA group and the negative control group (P>0.05). Compared with the negative control group (P<0.05), expression of Bip protein was increased in 0.02 mmol/L and 0.1 mmol/L FA group,the difference was statistically significant (P<0.05).CONCLUSION:Formaldehyde exposure may induce endoplasmic reticulum stress of hepatocytes.关键词
甲醛/内质网应激/肝细胞/免疫球蛋白重链结合蛋白/钙连蛋白Key words
formaldehyde/endoplasmic reticulum stress/hepatocytes/Bip/CANX分类
医药卫生引用本文复制引用
张超,白剑英,王东新,闫丹丹,王盼,刘艳飞..甲醛对人肝癌细胞株HepG2内质网应激相关Bip和CANX mRNA及蛋白表达水平的影响[J].癌变·畸变·突变,2017,29(5):340-345,351,7.基金项目
山西省回国留学人员科研资助项目(2014-034) （2014-034）
山西省留学回国人员科技活动择优资助项目(晋人社厅函〔2014〕376号) （晋人社厅函〔2014〕376号）
