癌变·畸变·突变2017，Vol.29Issue(5)：346-351,6.DOI:10.3969/j.issn.1004-616x.2017.05.005
阿胶对人工细颗粒物所致大鼠呼吸系统损伤的保护作用
Protective effects of Colla Corii Asini on artificial fine particulate matter-induced respiratory system injury in rats
摘要
Abstract
OBJECTIVE:To study the protective effects of Colla Corii Asini on respiratory system injury which was induced by artificial fine particulate matter (aPM2.5) in rats. METHODS:96 SD rats(half male and half female)were randomly divided into four groups,control,model,Colla Corii Asini low dose (0.3 g/kg) and high dose (1.2 g/kg) groups. Rats in both model and Colla Corii Asini groups were exposed to aPM2.515 minutes per day of 7 days a week for 8 weeks using the aerosol exposure system. In contrast,rats in the control group were exposed to clear air. 1 h before daily exposure to aPM2.5,rats of Colla Corii Asini groups were given Colla Corii Asini by intragastric administration. During the experimental period,the EMKA animal lung function monitoring system was used to monitor the lung function of rats. Every two weeks,blood serum and bronchoalveolar lavage fluid (BALF),and lung tissues were collected to detect changes in biomarkers of oxidative injury. RESULTS:Compared with the control group,the tidal volume (TV), expiratory volume (EV) and minute volume (MV) of the model group were markedly reduced,while the inspiratory time (Ti),expiratory time (Te) and relaxation time (RT) were significantly increased.,However,treating rats with Colla Corii Asini before aPM2.5 exposure significantly prevented the decline of TV,MV,EV and the increase of Ti,RT,Te. The lung phagocyte counts of the model group increased significantly compared with the control group,while the situation of Colla Corii Asini high dose group achieved a certain degree of relief. Compared with the control group,the concentration of methane dicarboxylic aldehyde (MDA) in serum and BALF of the model group increased,and with the treatment of Colla Corii Asini,the MDA decreased. Compared with the control group,the acvtivity of glutathione peroxidase (GSH-Px) in serum and BALF of the model group decreased,and with the treatment of Colla Corii Asini,the the acvtivity of glutathione peroxidase (GSH-Px) increased. CONCLUSION:Treatment of the model rats with Colla Corii Asini prevented decline of lung function and reduced lung phagocyte counts which were caused by aPM2.5,It is possible that Colla Corii Asini can prevent oxidative damage caused by aPM2.5. Further study is needed to address the mechanism for interactions.关键词
人工细颗粒物/阿胶/呼吸功能/氧化损伤Key words
artificial fine particulate matter/Colla Corii Asini/respiratory function/oxidative damage分类
医药卫生引用本文复制引用
张飘飘,凌亚豪,阎晓丹,魏金锋,靳洪涛,王爱平..阿胶对人工细颗粒物所致大鼠呼吸系统损伤的保护作用[J].癌变·畸变·突变,2017,29(5):346-351,6.基金项目
重大新药创制专项(2012ZX09301002-001-009) （2012ZX09301002-001-009）
