癌变·畸变·突变2017，Vol.29Issue(5)：358-363,6.DOI:10.3969/j.issn.1004-616x.2017.05.007
热休克蛋白HDJ1影响肺腺癌细胞侵袭和迁移能力的研究
Heat shock protein HDJ1 on invasion and migration ability of lung adenocarcinoma cells
摘要
Abstract
OBJECTIVE:To investigate expressions of HDJ1 in lung adenocarcinoma tissues and cell lines,and to reveal the role of HDJ1 in invasive ability of lung adenocarcinoma. METHODS:Immunohistochemistry method was used to detect expressions of HDJ1 in lung adenocarcinoma tissues and adjacent normal tissues. Western blot method was applied to determine expressions of HDJ1 in human lung adenocarcinoma cell lines (A549,H1299,H292) and normal lung epithelial cell line (Bease-2B). pMagic 7.1-HDJ1-KD lentivirus was transfected into the A549 cell line to construct a HDJ1 down-expressed cell line,and pMagic 7.1-NC lentivirus was transfected into the same cells as the control. The invasion ability of HDJ1 down-expressed group and control group was analyzed using the Transwell and the Wound Healing assays. RESULTS:The expression levels of HDJ1 in lung adenocarcinoma tissues were significantly higher than those in the adjacent normal tissues. HDJ1 was over-expressed in the A549 cell line. Compared with the control group, the Wound Healing assay results show that,the migration and healing ability of the HDJ1 down-expressed A549 cells were decreased. At 48 h after transfection,both the invasion and migration abilities of the HDJ1 down-expressed A549 cells were lower than that of control cells (P<0.05). CONCLUSION:Heat shock protein HDJ1 were highly expressed in the lung adenocarcinoma tissues and cell lines. In addition,HDJ1 promoted the invasion and migration ability of lung adenocarcinoma cells.关键词
热休克蛋白/HDJ1/肺腺癌/侵袭/迁移Key words
heat shock protein/HDJ1/lung adenocarcinoma/invasion/migration分类
医药卫生引用本文复制引用
李雨轩,李凯,张彤彤,郭元彪,殷俊,包勇..热休克蛋白HDJ1影响肺腺癌细胞侵袭和迁移能力的研究[J].癌变·畸变·突变,2017,29(5):358-363,6.基金项目
四川省应用基础研究计划项目(2014JY0136) （2014JY0136）
四川省科技厅基金(2014SZ0055) （2014SZ0055）
