癌变·畸变·突变2017，Vol.29Issue(5)：364-369,6.DOI:10.3969/j.issn.1004-616x.2017.05.008
异欧前胡素对体外人表皮黑素细胞酪氨酸酶和β-肌动蛋白表达的调节作用
Regulatory effects of isoimperatorin on expression of tyrosinase and β-actin in cultured human epidermal melanocytes
摘要
Abstract
OBJECTIVE:To investigate the effects of isoimperatorin on expression of tyrosinase and β-actin in cultured human epidermal melanocytes. METHODS:Human epidermal melanocytes were separated,purified and cultured in vitro. These cultures were randomly divided into control and isoimperatorin groups. Cells were cultured with M-254 medium. For the isoimperatorin group,25 μmol/L isoimperatorin in M-254 was added to the cultures. After 48 h of incubation,the morphology of melanocytes was observed under an inverted microscope. Melanin content was assayed by using the NaOH method. The methods of qPCR,Western blot and immunofluorescent were used to analyze the expression of tyrosinase and β-actin. RESULTS:After treatment with 25 μmol/L isoimperatorin for 48 h,dendrites of melanocytes were slightly elongated compared with the untreated cells. In addition,the treatment reduced melanin content (P<0.01). The results of qPCR,Western blot and immunofluorescence assay consistently indicated that isoimperatorin reduced tyrosinase mRNA and protein,and increased β-actin mRNA and protein. CONCLUSION:Cultured human epidermal melanocytes which were treated with 25 μmol/L isoimperatorin had reduced content of melanin. The observed effect was associated with inhibition of tyrosinase mRNA and protein,and with increased β-actin mRNA and protein.关键词
黑素细胞/异欧前胡素/酪氨酸酶/β-肌动蛋白Key words
melanocyte/isoimperatorin/tyrosinase/β-actin分类
医药卫生引用本文复制引用
詹标,孙贝蕾,罗思聪,庄鸿凯,黄鹏洲,曾懿,陈玮芳,陈玮莹..异欧前胡素对体外人表皮黑素细胞酪氨酸酶和β-肌动蛋白表达的调节作用[J].癌变·畸变·突变,2017,29(5):364-369,6.基金项目
教育部大学生创新训练项目(201510560032) （201510560032）
广东省中医药局项目(20142082) （20142082）
