安徽农业科学2017，Vol.45Issue(26)：6-10,5.
环境UV-B辐射增强对叶用杜仲林叶片光合作用与活性物质含量的影响
Effects of Elevated UV-B Radiation on Photosynthesis and Contents of Active Substance of Eucommia ulmoides Plantation
摘要
Abstract
[Objective] The aim was to study the effects of environmental ultraviolet (UV-B) radiation on the photosynthesis,active substance content and membrane lipid peroxidation of leaves of Eucommia ulmoides and provide theoretical basis for the ecological cultivation of Eucommia ulmoides. [ Method ] Taking five years old Eucommia ulmoides leaves under different ultraviolet radiation treatments [ CK: + 0; T1: +1. 40 kJ/(m2·d);T2:+2. 81 kJ/(m2·d)] as materials,with the single factor analysis of variance and correlation analysis method were used to study the variation content of photosynthetic parameters,photosynthetic pigment,aucubin,chlorogenic acid,genipin,geniposide,geniposide acid, flavonoids,total phenol,tannin and MDA,H2O2,O2·-. [Result] Elevated UV-B radiation significantly reduced the photosynthetic rate (Pn), stomatal conductance (Gs),intercellular CO2 concentration (Ci),transpiration rate (Tr) and chlorophyll a/b,and increased the content of chlo-rophyll a,chlorophyll b,carotenoid and total chlorophyll significantly;the content of total chlorophyll in T1 treatment was the highest [(1. 15 ± 0. 049) mg/g];the content of carotenoid in T2 treatment was the highest [(0. 0360 ± 0. 0016) mg/g];UV-B radiation significantly reduced the partial metabolites such as aucubin,geniposide acid,geniposide and chlorogenic acid content in the leaves of Eucommia ulmoides,and the maxi-mum change was 20. 74 times,61. 23 times,16. 16 times and 13. 63 times,respectively,and other secondary metabolites,genipin,flavonoids,total phenolics and tannin content were significantly increased. The contents of total phenol and tannin in T1 treatment were 1. 21 times,1. 11 times, 1. 34 times and 1. 37 times higher than that of CK treatment,respectively,and the contents of total phenol and tannin were (1. 2900 ± 0. 0230) and (17. 30 ± 1. 44) μg/g respectively in T2 treatment. UV-B radiation significantly increased the contents of MDA,H2 O2 and O2·-,the content of MDA and O2·- in T2 treatment was 39. 46％ and 70. 48％ higher than that in CK treatment group. The content of H2 O2 in T2 treatment was 43. 51％ higher than CK treatment group. [Conclusion] UV-B radiation significantly affects the photosynthetic parameters of Eucommia ulmoides leaves and the main active substances such as chlorogenic acid,aucubin,geniposide and geniposide,so the UV-B radiation intensity can be used to improve the economic benefit of Eucommia ulmoides.关键词
杜仲/UV-B辐射/净光合速率/茶多酚/绿原酸Key words
Eucommia ulmoides Oliv/UV-B radiation/Net photosynthetic rate/Tea polyphenol/Chlorogenic acid分类
农业科技引用本文复制引用
付金颖,杨超,李德文,唐中华,郭晓瑞,祖元刚..环境UV-B辐射增强对叶用杜仲林叶片光合作用与活性物质含量的影响[J].安徽农业科学,2017,45(26):6-10,5.基金项目
中央高校基本科研业务费用专项(2572015CA03) （2572015CA03）
林业公益性行业科研专项( 201504701 -2 ) （ 201504701 -2 ）
中央高校基本科研业务费专项(2572014EA01-02). （2572014EA01-02）
