安徽农业科学2017，Vol.45Issue(26)：11-14,4.
大孔树脂纯化红芸豆种皮花色苷
Purification of Anthocyanins from Seed Coat of Red Kidney Bean ( Phaseolus vulgaris L. ) by Macroporous Adsorption Resin
郝瑞林 1谭午芳 1董倩倩 1范建凤2
作者信息
- 1. 忻州师范学院生物系,山西忻州034000
- 2. 忻州师范学院化学系,山西忻州034000
- 折叠
摘要
Abstract
[Objective] The aim was to study purification of anthocyanins from seed coat of red kidney bean by macroporous adsorption resin. [Method] The static adsorption and desorption characteristics of macroporous adsorption resins including AB-8,D101,SP825,NKA-9,XAD-16 and HPD100 for anthocyanins were investigated and the purification technology of anthocyanins by AB-8 macroporou adsorption resin were opti-mized. And the HPLC technology were applied to analyze the purified. [Result] Resin AB-8 showed a satisfactory adsorption-desorption capacity for purification of anthocyanins from seed coat of red kidney bean. The optimized purification condition as the flow rate of adsorption solution was 3. 0 BV/h,and the optimized desorption solution was 100％ ethanol (modified to pH 1. 0 by hydrochloric acid) with the flow rate of 4. 5 BV/h. The purified powder owned a purity of 86. 05％ and HPLC method proved the purification was effective. [Conclusion] The developed method can be applied to the purification of anthocyanins from seed coat of red kidney bean.关键词
红芸豆/花色苷/大孔树脂/纯化/HPLCKey words
Red kidney bean/Anthocyanins/Mmacroporou adsorption resin/Purification/HPLC分类
轻工纺织引用本文复制引用
郝瑞林,谭午芳,董倩倩,范建凤..大孔树脂纯化红芸豆种皮花色苷[J].安徽农业科学,2017,45(26):11-14,4.
