安徽农业科学2017，Vol.45Issue(26)：15-17,20,4.
根瘤农杆菌生产琥珀酰多糖发酵条件的优化
Optimization of Fermentation Conditions for Succinoglucan Production by Agrobacterium tumefacien
摘要
Abstract
[Objective] The aim was to optimize the fermentation conditions for succinoglucan production by Agrobacterium tumefacien. [Method] We optimized the fermentation conditions for succinoglucan production by Agrobacterium tumefacien through orthogonal experiment and single-fac-tor test. [Result] The optimal medium consisted of 70 g/L sucrose,6 g/L yeast extract,10 g/L CaCO3,1. 5 g/L MgSO4 and 0. 025 g/L KH2PO4, and the optimal fermentation conditions included the incubation time of 4 d,inoculum amount of 2. 5％,initial pH of 7. 2,culture temperature of 30 ℃ and shaking speed of 250 r/min. Under these conditions,the yield of succinoglucan from Agrobacterium tumefacien was obviously improved to 18. 550 g/L,compared with the initial 8. 010 g/L. [Conclusion] The study can provide reliable basis for utilizing this Agrobacterium strain to make a further research into succinoglucan and to potentially produce succinoglucan in an industrial scale.关键词
根瘤农杆菌/琥珀酰多糖/发酵Key words
Agrobacterium tumefacien/Succinoglucan/Fermentation分类
农业科技引用本文复制引用
郑素娅,弋伊,王金霞,牛延宁,贾彩凤,金明飞,常忠义,高红亮..根瘤农杆菌生产琥珀酰多糖发酵条件的优化[J].安徽农业科学,2017,45(26):15-17,20,4.基金项目
国家自然科学基金青年项目(31100109). （31100109）
