安徽农业科学2017，Vol.45Issue(26)：106-108,128,4.
塞尼卡病毒A VP1基因遗传进化分析
Phylogenetic Analysis of VP1 Gene of Senecavirus A
摘要
Abstract
[Objective]To study the genetic variation of senecavirus A VP1 gene. [Method]The positive samples of SVV in swine with vesicular symptom from two farms of Guangdong were amplified by RT-PCR method. The sequencing and phylogenetic analysis was made on Senecavirus A VP1. [Result]The homology between VP1 gene of these two strains and Brazil,USA and Chinese historical isolates on GenBank was 92％ -99％. The homology between VP1 of these two strains and USA isolate SVV-001 in 2002 were 92. 8％ and 92. 7％ respectively. The highest homology of SVA VP1 gene with Chinese isolates CH-02-2015, CH-03-2015 and CH-04-2015 reached 99. 1％. Sequencing analysis results showed that the VP1 gene existed scatter point,which suggested that the virus had evolved. [Conclusion]The research results can provide references for further study and differential diagnosis of SVA.关键词
塞尼卡病毒A/PCR鉴定/VP1/遗传变异分析Key words
Senecavirus A/PCR identification/VP1/Genetic variation analysis分类
农业科技引用本文复制引用
杨彩娟,温肖会,任裕其,冼望强,刘暖华,谢乐新..塞尼卡病毒A VP1基因遗传进化分析[J].安徽农业科学,2017,45(26):106-108,128,4.基金项目
广东省技术交易体系与科技服务网络建设领域项目(2016A040401012,2015A030401067,2014B040404061). （2016A040401012,2015A030401067,2014B040404061）
