陈海雷 1沈妙娜 1黄燕 1黄倩雯 1刘勇 1方建培1
作者信息
- 1. 510120 中山大学孙逸仙纪念医院儿科血液室
- 折叠
摘要
Abstract
Objective To study the relationship between the morphology of bone marrow cells and the immunological classification in diagnosing children with leukemia.Methods A total of 105 children with leukemia treated at our hospital in 2016 were retrospectively analyzed;the epidemiological characteristics and the expression of leukocyte antigen were summarized.Results (1) The ratioes of male and female in ALL and AML were 2.13∶1 and 1.44∶1,respectively;the median onset age was 3 years old and 2 years old,respectively.(2) Among the 105 children with leukemia,22 were acute myeloid leukemia (AML),72 acute lymphoblastic leukemia (ALL),2 unclassification acute leukemia,4 chronic myeloid leukemia (CML),2 juvenile myelomonocytic leukemia (JMML),2 neuroblastoma (NB) bone marrow infiltration,and 1 lymphoma with bone marrow metastasis by FAB-classifying standard;among the 72 cases of acute and chronic leukemia immune,16 cases were acute myeloid leukemia (AML),47 acute B lymphocytic leukemia (B-ALL),4 acute T lymphocytic leukemia (T-ALL),2 chronic myeloid leukemia (CML),1 juvenile granulocyte monocyte nuclear cell leukemia (JMML),1 lymphoma with bone marrow metastasis,and 1 showed no obvious abnormalities by immunophenotyping.(3) The diagnostic coincidence rate between the immunophenotyping and morphological classification was 91.67％ (66/72),the partial coincidence rate was 2.78％ (2/72),and the innconsistent diagnosis rate was 5.55％ (4/72).(4) CD19,CD34,CD10,and HLA-DR were highly expressed in childhood B-cell acute lymphoblastic leukemia,with detection rates of 100.00％ (47/47),80.85％(38/47),89.36％ (42/47),and 100.00％(47/47),respectively;the expression rate of CD20 in childhood B-cell acute lymphoblastic leukemia was 12.77％ (6/47) and CD13 and CD33 expression rates were both 21.28％ (10/47).Conclusions Immunophenotyping plays an important role in the diagnosis of childhood leukemia,but it can not completely replace morphological analysis,and it can make up the deficiency of the conventional examination of bone marrow.关键词
儿童急性白血病/免疫分型/形态学分型Key words
Acute leukemia in children/Immunophenotyping/Morphological classification
