广西医科大学学报2017，Vol.34Issue(9)：1270-1273,4.DOI:10.16190/j.cnki.45-1211/r.2017.09.003
2种细胞冻存液对脐血单个核细胞冻存质量的影响
The influence of two different cryopreservation solution on mononuclear cells from cord blood
摘要
Abstract
Objective:To investigate the effect of two cryopreservation solution on mononuclear cells of cord blood and provide ideal cryopreservation solution based on reasonable theories.Methods:The total number of cord blood cells cryopreserved by two cryopreservation solution (Group 1:culture medium:autologous plasma:DMSO=5∶4∶1;Group 2:autologous plasma:DMSO=9∶1)were counted by cell counting instrument.Meanwhile,the cell loss rate of cord blood cells and hematopoietic differentiation ability of cord blood cells were respectively assessed by FACS and MethodCult assay.Results:The results from the cell count and trypan blue staining experiments showed the total cellular recovery of cryopreservation group 2 was (72.88 ± 12.24) ％,which was significantly higher than that of cryopreservation group 1 (56.65±9.34)％;flow cytometry data also indicated that the CD34+cell loss rate was as high as(35.90±2.56)％,and other cell loss rates like CD14+ monocytes neutrophils,CD4+ T,CD8+ T and CD3-CD56+ NK cell was (49.95 ± 3.68)％,(24.21 ± 2.35)％,(33.87 ± 3.21)％ and (35.18 ± 3.52)％,respectively.Whereas the proportion of other cells in cryopreservation group 2 was increased except CD4+ T and CD8+T cell loss which was(13.50± 1.25)％ and(24.67±3.72)％.Even the results of hematopoietic differentiation ability showed that the CFU-GM and BFU-E colony groups of cryopreservation group 2 were also significantly more than those of cryopreservation group 1.Conclusion:The cryopreservation effect of cryopreservation group 2 (autologous plasma:DMSO=9∶1)was better for cryopreservation of umbilical cord blood mononuclear cell.关键词
造血干细胞/脐血/单核细胞/冻存Key words
hemopoietin stem cell/umbilical cord blood/mononuclear cells/cryopreservation分类
医药卫生引用本文复制引用
黄馨萍,林科佳,魏宗科,魏志璋,罗晓玲,王宇环..2种细胞冻存液对脐血单个核细胞冻存质量的影响[J].广西医科大学学报,2017,34(9):1270-1273,4.基金项目
广东省科技计划项目(No.2014B09091036) （No.2014B09091036）
