广西植物2017，Vol.37Issue(9)：1187-1194,8.DOI:10.11931/guihaia.gxzw201612008
宁夏不同地区灵武长枣果实多糖的单糖成分分析
Analysis on monosaccharide composition of polysaccharides in Ziziphus jujube cv. Lingwuchangzao from different areas in Ningxia
摘要
Abstract
Fruits of Ziziphus jujube cv. Lingwuchangzao at mature stage from different areas in Ningxia(Lingwu, Yin-chuan, Zhongning, Qingtongxia) were used as the experimental materials. The water extraction and alcohol precipitation method were adopted to obtain polysaccharide, which was subsequently purified by DEAE-52 cellulose column and HW-55S gel column. Finally gas chromatography-mass spectrography( GC-MS) was used to analyze the monosaccharide com-position from purifed polysaccharides. The results showed that the crude polysaccharides from Lingwu had the highest yield, 1.795%. After purifed, all polysaccharides were fractioned respectively to one neutral fraction,Ju-0, and three acid fractions,Ju-1, Ju-2, Ju-3,and Ju-2 had the highest contents. This indicated that the main components of polysac-charides in Z. jujube cv. Lingwuchangzao were acid fractions. The results from GC-MS showed that Z. jujube cv. Ling-wuchangzao were composed of arabinose, rhamnose, ribose, fucose, xylose, mannose, galactose, glucose, glucuronic acid and galacturonic acid, but monosaccharide contents were different among different fractions. Arabinose, ribose, ga-lactose, glucuronic acid and galacturonic acid were the main components, and the content of xylose was the lowest. Both Ju-0 and Ju-1 were mainly composed by arabinose, ribose, galactose, while Ju-2 and Ju-3 exist obvious differences be-tween four areas. According to the comparision of the total contents of monosaccharides, the content of arabinose in poly-saccharides from Lingwu reached the highest, accounting for 28.6% of the total contents of monosaccharides, but other areas only accounted for 13%-18.9%. Meanwhile, glucuronic acid reached 21%-26.5% in polysaccharides from other three areas, but it was only 2.6%in polysaccharides from Lingwu. This indicates that the key difference in monosaccha-ride composition of polysaccharides from different areas lies in the content of arabinose and glucuronic acid. The advanta-ges of polysaccharides in Z. jujube cv. Lingwuchangzao from Lingwu mainly are high content of arabinose and low content of glucuronic acid, which is likely to be one of key factors of the difference in fruit quality. This study will provide basis and scientific support for further of development and utlization of the fruits of Z. jujube cv. Lingwuchangzao.关键词
灵武长枣/多糖/提取/分离纯化/单糖组成Key words
Ziziphus jujube cv. Lingwuchangzao/polysaccharides/extraction/purification/monosaccharide compositions分类
农业科技引用本文复制引用
柴雅红,章英才..宁夏不同地区灵武长枣果实多糖的单糖成分分析[J].广西植物,2017,37(9):1187-1194,8.基金项目
国家自然科学基金(31160057) [Supported by the National Natural Science Foundation of China(31160057)]. （31160057）
