河北医学2017，Vol.23Issue(9)：1455-1459,5.DOI:10.3969/j.issn.1006-6233.2017.09.014
LGR5在宫颈癌发生中的作用及机制初探
The Role and Mechanism of LGR5 in Cervical Carcinogenesis
摘要
Abstract
Objective:To investigate the role and mechanism of LGR5 in cervical carcinogenesis. Meth-ods:The expression of LGR5 and β-catenin in normal cervix, cervical intraepithelial neoplasias and squa-mous cervical cancer were determined by immunohistochemistry and western blot, and analyze the relationship between LGR5 and clinical pathological features of cervical cancer and β-catenin expression. The effect of LGR5 on cervical carcinogenesis was examined using tumorsphere formation. Results: The positive rate of LGR5 and β-catenin in normal cervix, cervical intraepithelial neoplasias and cervical cancer were 17％ ( 5/30), 65％ (11/17), 84％ (54/64) and 13％(4/30),53％(9/17),92％(59/64),respectively. The differ-ence was statistically significant ( P<0.01) . The expression of LGR5 was associated with pathological grade of cervical cancer, but not with patient's age, clinical stage and lymph node metastasis. β-catenin expression in cervical cancer was positively related to LGR5 expression(r=0.69, P<0.01). Moreover, the rate of tumor-sphere formation of LGR5 overexpression SiHa cell was significantly higher than the GFP control group ( P<0. 01) . Conclusion:LGR5 and promoted cervical carcinogenesis and its mechanismmay be associated with high expressio0n of β--catenin关键词
LGR5/宫颈癌/β-catenin/免疫组化/WesternblotKey words
LGR5/Cervical cancer/β-catenin/Immunohistochemistry/Western blot引用本文复制引用
陈庆,李向红,赵金燕,卢晓宁,薛翔..LGR5在宫颈癌发生中的作用及机制初探[J].河北医学,2017,23(9):1455-1459,5.基金项目
陕西省自然科学基金,(编号:2016JM8067) （编号:2016JM8067）
