郑州大学学报（医学版）2017，Vol.52Issue(5)：531-535,5.DOI:10.13705/j.issn.1671-6825.2017.05.004
食管鳞癌Eca109细胞中PI3K/AKT/mTOR信号通路对LSD1的调控作用
Regulation effects of PI3K/AKT/mTOR signaling pathway on LSD1 in Eca109 cells
摘要
Abstract
Aim: To explore the regulation effects of PI3K/AKT/mTOR signaling pathway on LSD1 in esophageal squamous cell carcinoma ( ESCC) Eca109 cells.Methods : Eca109 cells were treated with PI3K/AKT/mTOR signaling pathway inhibitor LY294002 or RAD001 with different concentrations for 24, 48 h, then the proliferation of cells was detec-ted by CCK-8 assay.Eca109 cells were treated with LY294002 or RAD001 with different concentration for 24 h,or treated with LY294002 or RAD001 at the same concentration for different time ,then the expressions of proteins in PI3KA/KT/mTOR signaling pathway, LSD1 and histone H3K4me2 were detected by Western blot, respectively.Results:The prolif-eration of Eca109 cells was significantly inhibited by LY 294002 or RAD001 , and the inhibition effects increased along with the increase of the concentration (P<0.05); LY294002 inhibited the expression of p-p70S6 K and promoted the expres-sions of Raptor, Rictor, p-Akt(Ser473) and histone H3K4me2;while RAD001 inhibited the expressions of Raptor , Ric-tor, p-p70S6K, up-regulated the expressions of p-Akt(Ser473) and histone H3K4me2, and down-regulated that of LSD1 (P<0.05).Conclusion:The PI3K/AKT/mTOR signaling pathway has regulation effects on LSD 1.关键词
食管鳞癌/PI3K/AKT/mTOR通路/LSD1/H3K4me2Key words
esophageal squamous cell carcinoma/PI3K/AKT/mTOR signaling pathway/LSD1/H3K4me2分类
医药卫生引用本文复制引用
凡丞,鲁照明,张幸丽,田菲,白一汝,赵琦,彭柯峥,刘宏民,侯桂琴..食管鳞癌Eca109细胞中PI3K/AKT/mTOR信号通路对LSD1的调控作用[J].郑州大学学报（医学版）,2017,52(5):531-535,5.基金项目
国家自然科学基金重点项目 81430085 （）
河南省基础与前沿技术研究计划项目 162300410122 （）
