郑州大学学报（医学版）2017，Vol.52Issue(5)：536-540,5.DOI:10.13705/j.issn.1671-6825.2017.05.005
HPK1在乳腺癌组织中的表达及HPK1过表达对人乳腺癌MCF-7细胞增殖、凋亡的影响
Expression of HPK1 in breast carcinoma tissue and effects of HPK1 over-expression on proliferation and apoptosis of human breast carcinoma cell MCF-7
摘要
Abstract
Aim:To detect the expression of hematopoietic progenitor kinase 1(HPK1) in carcinoma tissue of human in-vasive ductal breast carcinoma-not otherwise specified and breast carcinoma cells , and to investigate the effects of HPK 1 over-expression on proliferation and apoptosis of human breast carcinoma cells by constructing HPK 1 lentiviral vector .Methods:The expression level of HPK 1 protein in carcinoma tissue and paired adjacent tissue from 48 cases of breast carcinoma was de-tected by Western blot .The expression level of HPK1 in MCF10A and MCF-7 cells was detected by Western blot and RT-PCR.The HPK1 sequence was amplified by PCR , lentivirus recombinant vector pCDH-HPK1-puro was constructed , virus was packaged and MCF-7 cells were transfected ( overexpression group ) .The MCF-7 cells infected with empty vector virus was used as control group .The expression level of HPK 1 protein and mRNA was detected by Western blot and RT-PCR, respec-tively.The cell proliferation ability, cell apoptosis and cell cycle were detected by MTT assay and flow cytometry , respective-ly.Results:The expression level of HPK1 protein was lower in breast carcinoma tissue compared with adjacent tissue (P=0.036).The level of HPK1 protein and mRNA was lower in MCF-7 cells, compared with that of MCF10A cells(P<0.05). Compared with the control group, the expression level of HPK1 in the overexpression group was up-regulated, cell prolifera-tion ability was decreased significantly , while the apoptosis rate and the proportion of G 0/G1 were significantly increased ( P<0.05).Conclusion:The expression of HPK1 in both breast carcinoma tissue and cell line is low , and overexpression of HPK1 could inhibit proliferation and induce apoptosis and block the cells in G 0/G1 phase.关键词
HPK1/过表达/乳腺癌/MCF-7细胞/增殖/凋亡Key words
HPK1/overexpression/breast carcinoma/MCF-7 cell/proliferation/apoptosis分类
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王娇娇,赵杰,王留兴,范智蕊,李砺锋,丁显飞,周学良,杨子悦,袁博,许振涛,马丙钧..HPK1在乳腺癌组织中的表达及HPK1过表达对人乳腺癌MCF-7细胞增殖、凋亡的影响[J].郑州大学学报（医学版）,2017,52(5):536-540,5.基金项目
科技部科技惠民计划专项基金资助项目 2013GS410101 （）
河南省重大科技专项基金资助项目 151100310800 （）
