郑州大学学报（医学版）2017，Vol.52Issue(5)：544-549,6.DOI:10.13705/j.issn.1671-6825.2017.05.007
针对癌睾抗原PLAC1、PL2L60、MAGE-3的长肽疫苗设计及其免疫活性检测
Design and immune activity detection of long peptide vaccine targeted at cancer-testis antigen PLAC1, PL2L60 and MAGE-3
摘要
Abstract
Aim:To design long peptides based on CTL epitope prediction for cancer testis antigen PLAC 1, PL2L60 and MAGE-3, and to study the immune activity of the long peptides .Methods:The CTL epitope of PLAC1, PL2L60 and MAGE-3 were predicted using online databases , the long peptides with appropriate length in the epitope region was chosen , and then they were synthesized chemically and purified .Finally in vitro and in vivo activity experiments were used to verify if the long peptides had immune activity .Results: HLA-A2 restricted CTL epitopes for PLAC1, PL2L60 and MAGE-3 were predicted, and 7 long peptides were designed .The autopresentation, long peptide-pulsed DC ELISPOT assay and LDH results showed that long peptides PLAC1 P42-64, PL2L60 P169-191, MAGE-3 P101-122 and MAGE-3 P152-175 had better immune activity; the ELISA results in vivo showed that MAGE-3 P152-175 induced more IFN-γrelease in HLA-A2.1/Kb transgenic mice, in vivo LDH results showed that the spleen lymphocytes from HLA-A2.1/Kb transgenic mice induced by the long peptides PLAC1 P42-64, PL2L60 P169-191, MAGE-3 P101-122 and MAGE-3 P152-175 had higher killing rate on target cells .Conclusion:Four long peptides pointing to cancer testis antigen PLAC 1, PL2L60 and MAGE-3 have been successfully identified , which could be used as immunotherapy vaccine in future .关键词
肿瘤免疫/癌睾抗原/表位预测/长肽Key words
tumor immunity/cancer testis antigen/epitope prediction/long peptide分类
医药卫生引用本文复制引用
翟文杰,吴亚红,韩艳林,李国栋,陈真真,杜江峰,祁元明,高艳锋..针对癌睾抗原PLAC1、PL2L60、MAGE-3的长肽疫苗设计及其免疫活性检测[J].郑州大学学报（医学版）,2017,52(5):544-549,6.基金项目
国家自然科学基金资助项目 81373228 （）
81601448 （）
81571547 （）
