郑州大学学报（医学版）2017，Vol.52Issue(5)：553-558,6.DOI:10.13705/j.issn.1671-6825.2017.05.009
河南省维生素D受体基因rs2222570和rs731236位点多态性与结核病的易感性
Association of polymorphisms of rs 2222570 and rs731236 in vitamin D receptor gene with tuberculosis susceptibility in Henan Province
摘要
Abstract
Aim:To evaluate whether the vitamin D receptor ( VDR) gene polymorphism or its haplotypes might be as-sociated with tuberculosis susceptibility in Henan Han population .Methods:A total of 260 patients with tuberculosis were recruited, and 260 healthy control matched by sex and age were chosen .Genetic polymorphisms of rs2222570 and rs731236 in VDR gene were analyzed using PCR-SSCP.The data were analyzed by SPSS 19.0.The comparison of haplo-types were carried out by SHEsis online .The MDR 3.0.2 software was applied to analyze gene-environment interaction on the risk of tuberculosis susceptibility .Results:The variant genotypes of rs 2222570 in VDR gene were significantly associ-ated with the risk of tuberculosis , and the risk of individuals carrying wildtype genotype C /C decreased by 50%( adjusted OR=0.501,95%CI:0.343-0.709).The frequency of allele C in rs2222570 locus was significantly higher in control group compared with the tuberculosis group (adjusted OR=0.632, 95%CI:0.493-0.868).Whereas the mutant genotype C/C of VDR gene rs731236 polymorphism increased the risk of tuberculosis ( adjusted OR =2.001, 95%CI:1.838 -2.191).Haplotype analysis showed significant differences in the frequency of haplotype CT ,TT,and TC between the two groups[CT:OR(95%CI)=0.673(0.521-0.847), P<0.001; TT:OR(95%CI) =1.332(1.043 -1.711), P=0.032;TC:OR(95%CI)=6.561(2.366-18.143), P<0.001].The interaction of the four factor model , rs2222570, rs731236 of VDR genotypes polymorphisms ,smoking and the history of exposure to tuberculosis , might increase the risk of tuberculosis [OR(95%CI)=7.619(2.201-26.354), P<0.001].Conclusion: The polymorphism of rs2222570 and rs731236 in VDR gene are associated with the risk of tuberculosis , and there may be significant interaction between the rs2222570 ,rs731236 of VDR genotypes polymorphisms ,smoking and history of exposure to tuberculosis .关键词
结核病/维生素D受体基因/单核苷酸多态性/多因子降维法Key words
tuberculosis/vitamin D receptor gene/single nucleotide polymorphisms/multifactor dimensionality reduction分类
医药卫生引用本文复制引用
石洁,朱岩昆,郑丹薇,马晓光,王少华,李辉,邢进,吴彩霞..河南省维生素D受体基因rs2222570和rs731236位点多态性与结核病的易感性[J].郑州大学学报（医学版）,2017,52(5):553-558,6.基金项目
"十二·五"科技重大专项课题 2014ZX10003002 （）
河南省卫生计生委普通科技攻关项目 201702277 （）
