郑州大学学报（医学版）2017，Vol.52Issue(5)：558-562,5.DOI:10.13705/j.issn.1671-6825.2017.05.010
TCDD对大鼠睾丸间质细胞凋亡相关蛋白表达及ROS含量的影响
Effects of TCDD on expressions of apoptosis related proteins and ROS content in leydig cells of rats
摘要
Abstract
Aim:To explore the effects of TCDD on expressions of apoptosis related proteins and ROS content in leydig cells of rats.Methods:Leydig cells from rats were treated with different doses of TCDD (0,1,5,10,50 nmol/L) for 24 hours, and the proliferation inhibition rate of leydig cells was determined by MTT assay , ROS content in leydig cells was determined by DCFH-DA method, apoptosis was observed by confocal microscopy combined Annexin V -FITC/PI staining, and the expressions of Bax , Caspase-3 and Bcl-2 proteins were detected by Western blot .Results:With the increase of the dose of TCDD, the proliferation inhibition rate and ROS content showed a rising trend (P<0.05), the expressions of Bax and Caspase-3 increased whereas that of Bcl-2 protein decreased(P<0.05).Conclusion:TCDD could significantly induce apoptosis of rat leydig cells .关键词
二噁英/睾丸间质细胞/凋亡相关蛋白/大鼠Key words
TCDD/leydig cell/apoptosis related protein/rat分类
医药卫生引用本文复制引用
殷俊,谭雪梅,张秀丽,冀萌萌,陶宇昌,刘蔓蔓,余增丽..TCDD对大鼠睾丸间质细胞凋亡相关蛋白表达及ROS含量的影响[J].郑州大学学报（医学版）,2017,52(5):558-562,5.基金项目
国家自然科学基金资助项目 21577119 （）
