郑州大学学报（医学版）2017，Vol.52Issue(5)：562-566,5.DOI:10.13705/j.issn.1671-6825.2017.05.011
芦丁对大鼠心肌缺血再灌注损伤的保护作用
Cardioprotective effect of rutin on myocardial ischemia-reperfusion injury in rats
摘要
Abstract
Aim:To investigate the cardioprotective effect of rutin on myocardial ischemia-reperfusion injury in rats . Methods:Male SD rats were randomly allocated into Sham operation group , myocardial ischemia-reperfusion group ( MI/R group),rutin high dose group(RH group,80 mg/kg), rutin middle dose group(RM group,40 mg/kg), and rutin low dose group(RL group,20 mg/kg)(n=10).Left anterior descending coronary artery was ligated to establish the myocardial is-chemia-reperfusion injury model .Echocardiography evaluation of cardiac function after myocardial ischemia-reperfusion in-jury was performed.The area of myocardial infarction was determined .The myocardial enzymes(CK-MB, LDH, and AST) production and oxidation indexes (SOD, GSH and MDA) activity in serum and tissue were determined with enzyme-labeled instrument .The cell morphology was observed by HE staining .The expression of heme oxygenase-1 enzyme ( HO-1 ) was detected by Western blot .Results:Compared with the MI/R group, EF and FS in the rutin groups were significantly in-creased, the area of myocardial infaction and myocardial enzymes were significantly decreased , the production MDA was significantly decreased , the activity of SOD and the production of GSH significantly increased , and the expression of HO-1 in the rutin groups was markedly increased (P<0.05).Conclusion:Rutin could increase HO-1 expression, therefore pro-mote the endogenous antioxidant system against the myocardial ischemia-reperfusion injury in rats .关键词
芦丁/氧化应激/缺血再灌注损伤/血红素加氧酶-1/大鼠Key words
rutin/oxidative stress/ischemia-reperfusion injury/heme oxygenase-1/rat分类
医药卫生引用本文复制引用
赵一灿,张丽华,赵文,魏博,蒋友旭..芦丁对大鼠心肌缺血再灌注损伤的保护作用[J].郑州大学学报（医学版）,2017,52(5):562-566,5.基金项目
国家自然科学基金 81270270,81470524,81603114 （）
教育部博士点博导专项基金 20134101110013 （）
中国博士后科学基金2016M592316 （）
