郑州大学学报（医学版）2017，Vol.52Issue(5)：580-585,6.DOI:10.13705/j.issn.1671-6825.2017.05.016
非小细胞肺癌组织中MAGE-A3和MAGE-C2 mRNA的表达
Expressions of MAGE-A3 and MAGE-C2 mRNA in non-small cell lung carcinoma tissue
摘要
Abstract
Aim:To analyze the expressions of MAGE-A3 and MAGE-C2 mRNA in non-small cell lung cancer (NSCLC) tissue and the relationship between their expressions and clinical pathological characteristics .Methods:Tumor tissue was collected from 206 patients diagnozed as NSCLC .Real-time PCR was performed to detect the expressions of MAGE-A3 and MAGE-C2 mRNA.The correlation of clinicopathological characteristics (gender, age, histologic grading, pathological type , lymph node metastasis , clinical stage ) with the expressions of MAGE-A3 and MAGE-C2 mRNA in NSCLC tissue was evaluated .Then siRNA was used to down-regulate the expression of MAGE-A3 in A549 cells and the effects of MAGE-A3 knockdown on biology behavior of A 549 cells were observed .Results:The mRNA expression rates of MAGE-A3 and MAGE-C2 in NSCLC tissue were 73.3%(151/206) and 52.9%(109/206), respectively.The mRNA ex-pression of MAGE-A3 was associated with clinical stage and lymph node metastasis (P<0.05).However, there was no re-lation between MAGE-C2 mRNA expression and clinical characteristics (P>0.05).The mRNA coexpression of MAGE-A3 and MAGE-C2 was associated with age and clinical stage (P<0.05).MAGE-A3 knockdown led to decrease of sphere for-mation, invasion, and metastasis abilities of cells(P<0.001).Conclusion: MAGE-A3 and MAGE-C2 are expressed in NSCLC at high level;MAGE-A3 and MAGE-C2 might be potential target antigens for immunotherapy of NSCLC .关键词
非小细胞肺癌/MAGE-A3/MAGE-C2Key words
non-small cell lung cancer/MAGE-A3/MAGE-C2分类
医药卫生引用本文复制引用
马星,陈新峰,张超奇,乔亚敏,张毅,王丽萍..非小细胞肺癌组织中MAGE-A3和MAGE-C2 mRNA的表达[J].郑州大学学报（医学版）,2017,52(5):580-585,6.基金项目
国家自然科学基金资助项目 81171986,81271815 （）
科技部国家重点研发计划 2016YFC1303500 （）
