河南农业大学学报2017，Vol.51Issue(4)：481-486,6.
白花泡桐茎和叶差异表达的cDNA-SRAP分析
Differential expression analysis of stem and leaf in Paulownia fortunei by cDNA-SRAP
摘要
Abstract
The stem and leaf of Paulownia fortunei to analyze the differential gene expression with the method of cDNA-SRAP.Among the 64 pairs of primers,25 pairs of primers were screened with good amplification effect,and 36 differential fragments were detected.The repetition rate of the two times PCR amplification was about 66.8％.After being selected,cloned,and sequenced,23 fragments of the differential sequence were measured,and the length was between 400 and 1 700bp.According to the genomic database,sequence analysis showed that 16 fragments have significant homologous nucleotide sequence and 7 fragments with unknown function gene sequence has high homology.关键词
cDNA-SRAP/白花泡桐/差异片段/功能分析Key words
cDNA-SRAP/Paulownia fortunei/differential fragments/functional analysis分类
农业科技引用本文复制引用
茹广欣,周霜晴,朱秀红,刘小囡,王鋆瑞..白花泡桐茎和叶差异表达的cDNA-SRAP分析[J].河南农业大学学报,2017,51(4):481-486,6.基金项目
国家科技支撑计划专题(2013BAD01B06) （2013BAD01B06）
大学生创新创业训练计划(1402103020) （1402103020）
