河南农业大学学报2017，Vol.51Issue(4)：554-560,565,8.
AtPHO2的CRISPR/Cas9靶向敲除及其在拟南芥中功能分析
Gene knock out and functional analysis of AtPHO2 by CRISPR/Cas9-induced mutagenesis
摘要
Abstract
We constructed CRISPR/Cas9-mediated AtPHO2 knock-out vector,then transformed in Arabidopsis.In the transgenic descendants,three Atpho2 positive mutants were screened.In the transgenic mutant,the gene expression of AtPHO2 was significantly down-regulated,only one in ten of the wild type.The phosphorus contents in mutants and in the wild types were tested,the results of which revealed that Pi gradually accumulated in the new leaves with the aging process.The leaf phosphorus contents of the mutant Atpho2 were 2 ～ 4 times for the wild type.The results implied that more phosphorus accumulated in Atpho2 mutants for the phosphorus metabolism blocking.Additionally,AtPHO2 is probably associated with abiotic stress through regulating phosphorus metabolism and Arabidopsis senescence.关键词
拟南芥/基因组编辑技术/规律成簇短回文重复序列/泛素结合酶24/磷代谢Key words
Arabidopsis/genome editing technology/CRISPR/Cas9/UBC24/phosphorus homeostasis分类
生物科学引用本文复制引用
王钊辉,石超男,杨天啸,薛亚东,孙虎威,唐贵良,张战辉..AtPHO2的CRISPR/Cas9靶向敲除及其在拟南芥中功能分析[J].河南农业大学学报,2017,51(4):554-560,565,8.基金项目
省部共建小麦玉米作物学国家重点实验室项目(39990002) （39990002）
