河南农业科学2017，Vol.46Issue(8)：137-141,146,6.DOI:10.15933/j.cnki.1004-3268.2017.08.026
辽宁地区猪繁殖与呼吸综合征病毒分离株ORF5基因的遗传变异分析
Genetic Variation Analysis of ORF5 Gene of PRRSV Isolates in Liaoning Area
摘要
Abstract
In order to study the biological characteristics and the genetic variation of porcine reproductive and respiratory syndrome virus(PRRSV) ORF5 gene in Liaoning area between 2014 and 2015,the full sequence of PRRSV ORF5 gene was amplified by RT-PCR from the PRRSV positive swine,and the sequence alignment and genetic variation analysis were conducted by DNA Star.The results showed that the ORF5 gene of 8 PRRSV strains was 603 bp,which belonged to the American mutant strain.Compared with representative strains,the nucleotide and amino acid homology were 91.5％ to 99.0％ and 88.1％ to 98.5％,respectively.The genetic variation analysis suggested that the isolated strains were in different branches of evolution,but they were located in the subgroup 3.These results indicated that the main epidemic strain in Liaoning area between 2014 and 2015 was the highly pathogenic PRRSV,and various PRRSV strains existed in different regions of Liaoning at the same time,which had the same evolutionary origin.关键词
辽宁地区/猪繁殖与呼吸综合征病毒/ORF5/遗传变异Key words
Liaoning area/PRRSV/ORF5/genetic variation分类
农业科技引用本文复制引用
关淼,赵晓彤,曹东,顾贵波,杨本勇,李树博,申贯男,魏萍..辽宁地区猪繁殖与呼吸综合征病毒分离株ORF5基因的遗传变异分析[J].河南农业科学,2017,46(8):137-141,146,6.基金项目
辽宁省自然科学基金指导计划项目(20170540477) （20170540477）
辽宁省农业攻关项目(2015202013) （2015202013）
