湖南中医药大学学报2017，Vol.37Issue(9)：931-934,4.DOI:10.3969/j.issn.1674-070X.2017.09.003
密蒙花颗粒的急性毒性实验研究
Acute Toxicity Test of Flos Buddlejae Granules
摘要
Abstract
Objective To observe the acute toxic reaction of Flos Buddlejae granules and the situation of mice's death, to provide basis for the clinical safety od medication. Methods Having preliminary experiment on thirty mice with different concentrations of suspension liquid. There was no mice's death in 7 days, LD50 could not been detcted. Therefore, the maximum tolerance (MTD) was measured. Forty mice were randomly divided into the medicated group and the con-trol group. In line with the maximum volume of the mice, the maximal suspension concentration of the Flos Buddle-jae granules and the distilled water were separately given twice daily. Keeping the observation for 14 days, and recording the general condition and body mass in mice every day. After 14 days, the mice were killed. The blood routine indexes, and the major organs indexes of liver, spleen, kidneys and so on, the change of anatomy and pathological histology was observed. Results There was no mice death in medicated group. Compared with the control group, the body weight of mice in medicated group had no significant changes (P>0.05). The routine blood indexes in the medicated group were not signifi-cantly different (P>0.05). The maximum tolerance dose of Flos Buddlejae granules in mice was 114 g/kg daily. Conclusion The acute toxicity result shows that the clinical dose of Flos Buddlejae granules is safe.关键词
密蒙花颗粒/急性毒性试验/半数致死量/最大耐受量Key words
Flos Buddlejae Granules/Acute toxicity test/median lethal dose/maximum of tolerance dose分类
医药卫生引用本文复制引用
彭晓芳,王英,覃艮艳,张又玮,彭俊,彭清华..密蒙花颗粒的急性毒性实验研究[J].湖南中医药大学学报,2017,37(9):931-934,4.基金项目
国家自然科学基金面上资助项目(30772824,81574031) （30772824,81574031）
湖南省高层次卫生人才"225"工程培养项目资助 （）
湖南省科技厅科研基金资助项目(2015SF2016-6) （2015SF2016-6）
湖南省发展改革委科研基金资助项目(湘发改投资[2014]658 号) （湘发改投资[2014]658 号）
长沙市科技计划重大专项(K1501014-31) （K1501014-31）
湖南中医药大学研究生创新课题项目(2016CX19). （2016CX19）
