渔业科学进展2017，Vol.38Issue(5)：164-170,7.DOI:10.11758/yykxjz.20160525001
基于钨丝原子阱与直接进样技术的原子荧光光谱法快速测定贝类中的镉
Rapid Determination of Cadmium in Shellfish Based on Direct Sampling Electrothermal Vaporization Atomic Fluorescence Spectrometry with a Tungsten Coil Trap
摘要
Abstract
Cadmium is among the most toxic metals in the aquatic environment.It particularly affects shellfish because the latter can accumulate large amounts of cadmium from the water.To reduce the potential risk on the health of public consumers,it is necessary to measure and monitor the level of cadmium in shellfish.Conventional analysis methods are usually time-consuming due to the prolonged sample digestion and pretreatments.A direct solid sampling technology without digestion treatment has been applied in the fast determination of cadmium concentration in agricultural produces such as grains and vegetables.Here,we tried to employ this method in the rapid measurement of cadmium concentration in shellfish.Solid sampling (SS) device was coupled with atomic fluorescence spectrometry (AFS),and the SS-AFS instrument was assembled with a tungsten coil trap (TCT) and porous carbon ETV.Meanwhile,electrothermal vaporization atomic fluorescence spectrometry (ETV-AFS) with a tungsten coil trap was optimized,and SS-ETV-AFS was established as a novel direct sampling method of detecting cadmium in shellfish.The results showed that a tungsten coil could work as a cadmium trap and eliminate most of the matrix interferences.Samples were homogenized and diluted with different acids,and 1％ HNO3 was determined to be the optimal acid reagent.Under the optimized conditions,the fitting with both the standard addition method and the standard curve method showed good linearity with regression coefficient (R2) larger than 0.995.The relative standard deviation (RSD) of the standard addition method and the standard curve method were 6.5％-7.7％ and 3.3％-8.6％ respectively;the spiked recoveries of them were 96.4％-105.5％ and 98.1％-110.6％;the limit of detection (LOD) were 0.6 pg and 3 pg.Moreover,the time elapsed (including sample pretreatments) was within 5 min.The standard addition method generated data with similar accuracy to those measured with graphite furnace atomic absorption spectrophotometry (GFAAS) or certified reference materials (CRMs) (P ＞ 0.05).Therefore,the standard addition method could be suitable to the field analysis and monitoring of cadmium in shellfish.关键词
固体进样/原子荧光/贝类/镉/测定Key words
Solid sampling/Atomic fluorescence spectrometry/Shellfish/Cadmium/Determination分类
轻工纺织引用本文复制引用
徐正,翟明丽,翟毓秀,冯礼,刘霁欣,尚德荣,赵艳芳,宁劲松..基于钨丝原子阱与直接进样技术的原子荧光光谱法快速测定贝类中的镉[J].渔业科学进展,2017,38(5):164-170,7.基金项目
国家重大科学仪器设备开发专项(2011YQ14014906)和国家自然科学基金(41206101)共同资助[This work was supported by the National Key Scientific Instrument and Equipment Development Project (2011YQ14014906),and the National Natural Science Foundation of China (41206101)] （2011YQ14014906）
