林业工程学报2017，Vol.2Issue(5)：46-50,5.DOI:10.13360/j.issn.2096-1359.2017.05.009
EGCG-Cu络合物与牛血清蛋白相互作用研究
Study on binding of EGCG-Cu complex to bovine serum albumin
摘要
Abstract
Epigallocatechin-3-gallate (EGCG), the major polyphenolic constituent of green tea, has a variety of bio-logical functions in vitro, including the inhibition of cell proliferation, induction of cell cycle arrest or apoptosis, and inhibition of tumor angiogenesis and vascular tumor growth. The potential chelating activity of EGCG for metal ions, especially transitional metal ions, has significant effects on the bioactivity of EGCG. Binding interactions between EGCG and its copper complex and bovine serum albumin (BSA) were investigated through fluorescence spectroscopy in this study. The effect of copper on the interaction between EGCG and BSA was also analyzed. The different quenching mechanisms in the binding interaction were studied and the binding constants of EGCG, EGCG-Cu(Ⅱ) to BSA were obtained by using different modified Stern-Volmer equations. The results showed that, when EGCG and EGCG-Cu(Ⅱ) were added to the BSA solution, the fluorescence was quenched, indicating that interactions happened between EGCG, EGCG-Cu(Ⅱ) and BSA. The fluorescence intensity gradually decreased with the increasing concen-tration of EGCG with an obvious red-shift of the maximum of BSA. When EGCG was continuously added to BSA so-lution containing Cu(Ⅱ) at pH 7. 4, the fluorescence of BSA decreased. There was no spectral shift when the EGCG was added to BSA containing Cu(Ⅱ), indicating that the mode of EGCG-BSA interaction is expected to be altered by Cu(Ⅱ). Additionally, the binding constant between EGCG and BSA increased significantly after EGCG was mod-ified by Cu(Ⅱ) to form EGCG-Cu(Ⅱ) complex. Our results suggest that after chelating with copper, the interaction mode of EGCG with BSA is altered and this could have various implications on its other physicochemical activities.关键词
表没食子儿茶素没食子酸酯(EGCG)/牛血清白蛋白(BSA)/荧光猝灭法/Stern-Volmer方程Key words
EGCG/BSA/fluorescence spectroscopy/Stern-Volmer equation分类
生物科学引用本文复制引用
张亮亮,徐曼,胡新宇,汪咏梅..EGCG-Cu络合物与牛血清蛋白相互作用研究[J].林业工程学报,2017,2(5):46-50,5.基金项目
国家重点研发计划(2017YFD0601000) （2017YFD0601000）
江苏省生物质能源与材料重点实验室基本科研业务费项目(JSBEM-S-201707). （JSBEM-S-201707）
