四川大学学报（自然科学版）2017，Vol.54Issue(4)：870-873,4.DOI:10.3969/j.issn.0490-6756.2017.04.034
见血青总生物碱凝血活性的研究
Study on coagulation activity of total alkaloids in Liparis nervosa (Thunb.) Lindl.
摘要
Abstract
A solution (TASLN) of 10mg/ml total alkaloids of Liparis nervosa (TALN) was prepared,which was extracted by Ultrasonic-assisted method.Normal saline (NS) was used as blank control and Yunan Baiyao as control in this present study.For studing the blood coagulation in vivo,we measured the bleeding time and the amount of bleeding of mice.For coagulation in vitro,the hemostasis time of wound was recorded of rabbits.The rabbits' anticoagulant blood was used to measure the coagulation time,prothrombin time (PT) and recalcification time (RT),respectively.The results showed that the time and the amount of mice bleeding was 212.95s and 42.5 square.The coagulation time was 192.23s by blood congeals method and 245.48s by tube method,PT and RT of rabbits' blood of TALN was 12.22s and 180.78s,all the times were shorter than that of blank control.The average hemostasis time of total alkaloids solution of L.nervosa (81.50s) was obviously shorter than that of NS (125.20s),but showed no significant difference with that of Yunnan Baiyao (75.20s).The TALN shows obvious coagulation activity.关键词
见血青/总生物碱/凝血活性Key words
Liparis nervosa/Alkaloids/Coagulation activity分类
农业科技引用本文复制引用
张杨,樊晓旭,柴淑丽,王丽..见血青总生物碱凝血活性的研究[J].四川大学学报（自然科学版）,2017,54(4):870-873,4.基金项目
四川省科技厅科技培训计划项目(2015KZ0003) （2015KZ0003）
