山东中医杂志2017，Vol.36Issue(10)：893-897,5.
星点设计-效应面法优化纤维素酶法辅助提取鬼针草叶中总黄酮研究
Optimized Cellulase Assisted Extraction of Total Flavonoids from Bidens pilosa Linn. Leaves by Central Composite Design and Response Surface Method
摘要
Abstract
Objective:To extract total flavonoids from Bidens pilosa Linn. leaves through optimized cellulose process. Methods:Based on single-factor experiments,three factors of the enzyme amount,pH value,enzymoly-sis temperature were chosen to optimize the cellulase assisted extraction total flavonoids from Bidens pilosa Linn. leaves by central composite design and response surface method. Results:The best extraction process was as follows:the enzyme amount 1.14％,enzymolysis temperature 41.9℃,pH value 4.35;the optimized ex-traction rate of total flavonoids was 6.31％,which was different from the model prediction by 1.25％. Conclu-sion:Central composite design and response surface method was suitable for the extraction process optimiza-tion of cellulose assisted extraction total flavonoids from Bidens pilosa Linn. leaves. The established mathe-matical model for the predictability was satisfactory.关键词
鬼针草/纤维素酶/总黄酮/星点设计/响应面法Key words
Bidens pilosa Linn./cellulose/total flavonoids/central composite design/response surface method分类
医药卫生引用本文复制引用
张玲,和焕香,刘武,夏真真,周洪雷..星点设计-效应面法优化纤维素酶法辅助提取鬼针草叶中总黄酮研究[J].山东中医杂志,2017,36(10):893-897,5.基金项目
山东省科技发展计划项目(编号:2013GSF11903) （编号:2013GSF11903）
山东中医药大学 2015年度大学生研究训练计划(SRT)项目(编号:2015130) （SRT）
