生物加工过程2017，Vol.15Issue(5)：86-92,7.DOI:10.3969/j.issn.1672-3678.2017.05.011
一碳化合物利用新途径的设计和体外构建
A computationally designed pathway for one carbon compounds utilization and its in vitro construction
摘要
Abstract
C1 compounds can be widely used in industrial biotechnology as low-cost alternative substrates to sugars.In normal pathways for the synthesis of acetyl coenzyme A derived products from C1 compound, one third of the carbon atoms from the substrate is wasted as CO2 ,and thus the product yield is low. Therefore,it is important to find new carbon conserved synthesis pathways for acetyl-CoA derived products from C1 compounds.In this study,we built a large metabolic network based on reactions from MetaCyc database and used the flux balance analysis method to find a new pathway from formaldehyde to acetyl coenzyme A derived products.The new pathway is carbon conserved and ATP independent.We screened and purified the enzymes in the pathway and tested the pathway in vitro using acetate as the target product.The concentration of acetate produced from the system is enhanced with the increased addition of formaldehyde substrate.The carbon molar yield can achieve 90％ under certain conditions,exceeding the theoretical yield of 67％ from naturally existing pathways.关键词
代谢网络模型/一碳化合物/乙酰辅酶A/新途径/体外验证Key words
metabolic networks/C1 compounds/acetyl coenzyme A/new pathway/in vitro experiment分类
生物科学引用本文复制引用
陈阳,杨雪,袁倩倩,罗浩,李培顺,马红武..一碳化合物利用新途径的设计和体外构建[J].生物加工过程,2017,15(5):86-92,7.基金项目
天津科技支撑项目(14ZCZDSY00060) （14ZCZDSY00060）
中国科学院重点研究项目(ZDRW-ZS-2016-3) （ZDRW-ZS-2016-3）
