现代电子技术2017，Vol.40Issue(18)：1-6,6.DOI:10.16652/j.issn.1004-373x.2017.18.001
液滴数字PCR芯片结果自动化读出平台的研究
Research on automatic result readout platform of droplet digital PCR chip
摘要
Abstract
As the third generation PCR technique,the droplet digital PCR(ddPCR)can realize the absolute quantification for nucleic acid molecule,but in the process of PCR result readout,the statistics of ten-million magnitude droplets wastes time and manpower,and is easy to result in errors. Therefore,an automatic scanning and recognition platform of ddPCR chip was de-signed. The system includes the hardware mobile platform,and has the functions of software photographing,image splicing and droplet recognition. The sequential movement of the platform and photographing of camera are controlled by the software,and then the photographed pictures are spliced into a photograph to recognize droplets in it. The recognition rate of the platform for fluorescent and non-fluorescent droplets can reach up to above 99.6% by means of the accurate positioning and scanning,image segmentation and recognition algorithms of the 2D translation platform. This platform has been applied to the laboratory,can rec-ognize the microdroplets produced by digital PCR chip with one key,shorten the recognition time and reduce the recognition er-ror rate greatly.关键词
ddPCR/核酸分子定量/自动扫描/液滴识别Key words
ddPCR/nucleic acid molecule quantification/automatic scanning/droplets recognition分类
信息技术与安全科学引用本文复制引用
刘松生,袁浩均,刘强,周洪波,毛红菊,任伟..液滴数字PCR芯片结果自动化读出平台的研究[J].现代电子技术,2017,40(18):1-6,6.基金项目
国家自然科学基金项目(21275153 （）
61302052 （）
61571428) （）
上海市浦江人才项目(15PJ1409800) （15PJ1409800）
