中国组织工程研究2017，Vol.21Issue(26)：4155-4159,5.DOI:10.3969/j.issn.2095-4344.2017.26.010
氧化锆氧化铝陶瓷复合材料对人牙周膜细胞增殖、凋亡和骨诱导活性的影响
Effects of zirconia/alumina ceramic composite on proliferation, apoptosis and bone induction activity of human periodontal ligament cells
摘要
Abstract
BACKGROUND: Zirconia/alumina ceramic composite with excellent corrosion resistance, high strength and toughness has wide application prospect.OBJECTIVE: To investigate the effect of zirconia/alumina ceramic composite on proliferation, apoptosis and bone induction activity of human periodontal ligament cells.METHODS: Human periodontal ligament cells at passages 3-5 were cultured routinely as control group and co-cultured with zirconia/alumina ceramic composite as experimental group. After 1-5 days of culture, MTT,TUNEL, immunohistochemical detection, and alkaline phosphatase staining were used to detect cell proliferation,apoptosis, type I collagen expression and osteogenic activity, respectively.RESULTS AND CONCLUSION: Within 1-5 days of culture, there were no differences in cell proliferation, apoptosis and alkaline phosphatase activity between the two groups. At 5 days of culture, the type I collagen expression showed no difference between the two groups. Taken together, the zirconia/alumina ceramic composite has no effect on the proliferation, apoptosis and bone induction activity of human periodontal ligament cells.关键词
生物材料/口腔生物材料/氧化锆氧化铝陶瓷复合材料/牙周膜细胞/生物相容性分类
医药卫生引用本文复制引用
王佳宁..氧化锆氧化铝陶瓷复合材料对人牙周膜细胞增殖、凋亡和骨诱导活性的影响[J].中国组织工程研究,2017,21(26):4155-4159,5.基金项目
吉林省教育厅"十三五"社会科学研究项目(吉教科文合字[2016]第30号):利用TDS系统对口腔修复教学的探索与研究 the Social Science Research Project of Jilin Provincial Education Department during the Thirteenth Five-Year Period, No. [2016]30 （吉教科文合字[2016]第30号）
