新疆农业科学2017，Vol.54Issue(6)：1076-1084,9.DOI:10.6048/j.issn.1001-4330.2017.06.014
新疆红枣枣果主要致病菌交链格孢菌产生毒素种类及黑斑病病果毒素含量测定
Mycotoxin Analysis of Main Pathogen Alternaria alternate of Jujube Fruit Shrink and Determination of Black Spot Disease Toxin Content in Xinjiang
摘要
Abstract
[Objective] In order to make clear mycotoxins species and content of mainly pathogen Alternaria alternate of jujube fruit shrink,jujube black spot,and mycotoxins species and content of jujube black spot diseased fruits,this study aims to provide the basis for the safety evaluation of jujube processing and consumption in Xinjiang.[Method] Utra-performance liquid chromatography-tandem mass spectrometry was established as the method to determine the variation and content of mycotoxins from 10 Alternaria strains and the onset fruit of jujube black spot in different severities and the species and content of toxin in artificially inoculated fruits.[Result]The results showed that 10 Alternaria strains could produce 4 kinds of Ahernaria mycotoxins:alternariol (AOH),alternariol monomethylether (AME),altenuene (ALT) and tenuazonic acid (TeA).TeA,ALT,AME and AOH were detected in different disease onset of jujube fruit which were affected by A.alternate and artificial cultivation conditions,the highest content of TeA,range of 3.1 × 103-5.5 × 103 mg/kg;AME,AOH and ALT were 7.2 × 102-6.4 × 102 mg/kg,1.2-3.8 × 102 mg/kg and 0.09-5.08mg/kg.After inoculation with A.alternaria,alarge number of TeA were not only detected injuring jujube fruit,but also detected in the uninjured jujube fruit.Not only the toxin was detected in the onset jujube fruit,but also a large amount of toxin was found in the jujube without injury,although there was a small amount of Alternaria toxin in healthy jujube fruit.[Conclusion] The main pathogens (Alternaria alternate) leading to jujube fruit shrink and jujube black spot in Xinjiangcan produce a lot ofAlternaria mycotoxins,which have such great influence on the production and value of Xinjiang red jujube that it was inedible.Therefore,it should be paid more attention to in fresh jujube processing and risk assessment,and to reduce the infection of pathogens in jujube planting and storage,so as to avoid more pollution.So in jujube planting and storage period,the pathogen infection must be reduced in order to avoid more contamination.关键词
红枣/枣果缩果病/枣果黑斑病/链格孢霉/毒素Key words
jujube/short ultivars/growth stages/major gene plus polygene inheritance/genetic analysis分类
农业科技引用本文复制引用
何丽,郭开发,艾尼古丽·依明,赵思峰..新疆红枣枣果主要致病菌交链格孢菌产生毒素种类及黑斑病病果毒素含量测定[J].新疆农业科学,2017,54(6):1076-1084,9.基金项目
The National Spark Program of China "Demonstration and Popularization of Green Prevention and Control Technology for Main Diseases and Pests of Jujube in Southern Xinjiang" (2015GA891007)国家星火计划“南疆红枣主要病虫害绿色防控技术示范与推广”(2015GA891007) （2015GA891007）
