厦门大学学报（自然科学版）2017，Vol.56Issue(5)：659-664,6.DOI:10.6043/j.issn.0438-0479.201607025
α-溴代肉桂醛对酪氨酸酶的抑制动力学及其杀虫作用
Anti-tyrosinase Activity Kinetics and Insecticidal Efficacy ofα-Bromocinnamaldehyde
摘要
Abstract
The inhibitory effect ofα-bromocinnamaldehyde on mushroom tyrosinase was investigated in this study using enzyme ac-tivity staining and enzyme inhibitory kinetics.The rate constants of the forward (k+0 )and reverse (k-0 )reactions were determined to be 2.793 L/(mmol·min)and 1.98×10-3 s-1 ,respectively.Moreover,the insecticidal efficacy ofα-bromocinnamaldehyde was de-termined.As expected,the larval growth and survival rate of Helicoverpa armigera were found to be reduced after supplemented with 0.625 mmol/Lα-bromocinnamaldehyde as compared to the control larvae,and the larvae were totally dead after 48 h treatment with 5.0 mmol/Lα-bromocinnamaldehyde.The polyphenol oxidase (PPO)activities were inhibited by 5.0 mmol/Lα-bromocinnam-aldehyde both in vivo and in vitro.These results may provide theoretical and practical basis in the application ofα-bromocinnamalde-hyde in food,agriculture and medicine.关键词
α-溴代肉桂醛/酪氨酸酶/棉铃虫Key words
α-bromocinnamaldehyde/tyrosinase/Helicoverpa armigera分类
生物科学引用本文复制引用
崔沂,胡泳华,石艳,陈清西,王勤..α-溴代肉桂醛对酪氨酸酶的抑制动力学及其杀虫作用[J].厦门大学学报（自然科学版）,2017,56(5):659-664,6.基金项目
国家自然科学基金(31271952) （31271952）
