厦门大学学报（自然科学版）2017，Vol.56Issue(5)：665-670,6.DOI:10.6043/j.issn.0438-0479.201609043
乳酸对东方肉座菌β-葡萄糖苷酶的抑制动力学
Inhibitory Kinetics by Lactic Acid onβ-glucosidase from Hypocrea orientalis
摘要
Abstract
The kinetics of inhibition ofβ-glucosidase purified from Hypocrea orientalis by lactic acid has been studied.The results show that the enzyme can be reversiblely inhibited by lactic acid with IC50 of 0.19 mol/L,while at low concentration the inhibition of the enzyme is a slow,competitive type reaction and the inhibition constant (KI)is 0.149 mol/L.The microscopic rate constants for the reactions of lactic acid with the enzyme have been determined and the values of forward (k+0 )and reverse (k-0 )inhibition con-stants are 0.4 L/(mol·min)and 0.056 min-1 ,respectively.The presence of the substrate has showed an obvious protective effect on the enzyme against the inhibition by lactic acid.Our study provides the theoretical support for lactic acid production industry by simultaneous saccharification-fermentation of cellulose.关键词
东方肉座菌/β-葡萄糖苷酶/乳酸/抑制动力学/发酵产物Key words
Hypocrea orientalis/β-glucosidase/lactic acid/inhibition kinetics/fermentation products分类
生物科学引用本文复制引用
许鑫琦,郎斌,邬小兵,林娟,陈清西..乳酸对东方肉座菌β-葡萄糖苷酶的抑制动力学[J].厦门大学学报（自然科学版）,2017,56(5):665-670,6.基金项目
国家海洋局海洋公益性项目(201305015) （201305015）
福州大学校人才基金(XRC-1551) （XRC-1551）
