厦门大学学报（自然科学版）2017，Vol.56Issue(5)：671-678,8.DOI:10.6043/j.issn.0438-0479.201612022
一株新型花生四烯酸生产菌株高山被孢霉LU166的形态调控及维生素培养优化
Morphology Control and Vitamins Optimization of a Novel Arachidonic Acid-producing Strain Mortierella alpina LU166
摘要
Abstract
An arachidonic acid (ARA)-producing strain Mortierella alpina LU166 ,which is independently isolated by our lab,was studied in the present work.Firstly,it's found that the mycelial morphology can be steadily kept as fluffy small pellets when cultured in baffled flask,which is more favorable for the accumulation of total lipids and ARA than that by filaments and big pellets morpho-logy.Then,the concentration of B-group vitamins (VB1 ,VB12 ,biotin and calcium pantothenate)was optimized to enhance the lipid production.When adding the combination of vitamins with the optimized concentration into the culture,the biomass,lipids and ARA yield reached 24.74,12.66 and 6.46 g/L,respectively,which increased by 10％,30％ and 16％,compared to the control group.关键词
高山被孢霉/花生四烯酸/凹槽摇瓶/B族维生素Key words
Mortierella alpina/arachidonic acid(ARA)/baffled flask/B-group vitamins分类
生物科学引用本文复制引用
刘晓婷,杨瑞琪,卢英华,凌雪萍..一株新型花生四烯酸生产菌株高山被孢霉LU166的形态调控及维生素培养优化[J].厦门大学学报（自然科学版）,2017,56(5):671-678,8.基金项目
厦门市科技计划项目(3502Z20153005) （3502Z20153005）
厦门南方海洋研究中心项目(15GYY024NF03) （15GYY024NF03）
