厦门大学学报（自然科学版）2017，Vol.56Issue(5)：686-692,7.DOI:10.6043/j.issn.0438-0479.201611008
复方中草药对白斑综合征病毒感染下凡纳滨对虾免疫活性的影响
Effects of Compound Chinese Herbs on Immune Activities of Litopenaeus vannamei After Infected with White Spot Syndrome Virus
摘要
Abstract
To study effects of compound Chinese herbs (including Radix acutellariae,R.puerariae,Coptidis rhizome,Lonicera japonica,R.isatidis,Astragalus radix,R.glycyrrhize,R.bupleuri,Angelica sinensis,Crataegus pinnalifida,Pericarpium Cit-rusa reticulatae and Poria cocos)on immunologic factors and white spot syndrome virus (WSSV)-resistant capacity of Litopenaeus vannamei,shrimps were fed 28 d with basal diets supplemented with 0 (control group),0.4％,0.8％,1.2％,1.6％,and 2.0％ of compound Chinese herbs,respectively.Immunologic factors and death status were determined and observed at 0,12,24,48 and 96 h after infected with WSSV.The results showed that activities of superoxide dismutase (T-SOD),alkaline phosphatase (AKP),acid phosphatase (ACP)and glutamic-oxalacetic transaminase (GOT)firstly increased and then decreased during 0-96 h,which reached a peak during 24-48 h after infected with WSSV.Meanwhile,the activities of the four immunologic enzymes above as well as glutamic-pyruvic transaminase (GPT)of the 0.8％ and 1.2％ supplemental groups were kept at a high level and remained steady during the whole experiment.Moreover,the content of malonaldehyde (MDA)of the 0.8％ and 1.2％ supplemental groups was significantly lower than that in other groups in 24-48 h (p<0.05).After 192 h infected with WSSV,the highest survival rate (33.33％)and 50％death time (150.50±48.88 h)appeared in the 0.8％ supplemental group,while the earliest 50％ death time and the fastest 100％death rate of shrimps appeared in the control group and 0.4％ supplemental group.In conclusion,compound Chinese herbs can pro-mote immunity performance and WSSV-resistant ability of L.vannamei,among which the 0.8％ supplemental groups showed the best effect.These results will provide scientific basis for reasonable use of the compound Chinese herbs for the healthy culture of L. vannamei.关键词
凡纳滨对虾/白斑综合征病毒/复方中草药/免疫反应Key words
Litopenaeus vannamei/white spot syndrome virus (WSSV)/compound Chinese herbs/immune response分类
农业科技引用本文复制引用
陈辉辉,涂晨凌,唐杨,黄永春..复方中草药对白斑综合征病毒感染下凡纳滨对虾免疫活性的影响[J].厦门大学学报（自然科学版）,2017,56(5):686-692,7.基金项目
福建省科技厅产学研项目(2017N51012) （2017N51012）
厦门市海洋经济创新发展区域示范项目(13PZY001SF25) （13PZY001SF25）
