西南国防医药2017，Vol.27Issue(9)：916-918,3.DOI:10.3969/j.issn.1004-0188.2017.09.002
HPLC法同时测定生脉散配方颗粒中人参皂苷Rg1和Re的含量
Simultaneous determination of ginsenoside Rg1 and Re in Shengmaisan Formula Granule by HPLC
摘要
Abstract
Objective To explore the feasibility of high performance liquid chromatography (HPLC) in the simultaneous determination of ginsenoside Rg1 and ginsenoside Re in Shengmaisan Formula Granule.Methods D101 macroreticular resin was used to separate and purify Shengrnaisan Formula Granule solution to prepare test solution.HPLC conditions were:Diamonsil TM C18 chromatographic column(5 μm,250 mm× 4.6 mm),mobile phase acetonitrile-0.05％ phosphoric acid solution(20∶80),flow velocity one ml/min,detection wavelength 203 nm and sample size 10 μl.Results The linear relation of ginsenoside Rg1 was good within the range of 0.252～2.52 μg (r=0.9998);the average recovery rate was 100.45％ (RSD=1.01％).The linear relation of ginsenoside Re was good within the range of 0.249～2.49 μg (r=0.9995);the average recovery rate was 98.77％ (RSD=1.22％).Conclusion The simultaneous determination of ginsenoside Rg1 and Re in Shengmaisan Formula Granule by HPLC is simple and highly accurate and repeatable and may be used in the quality control of Shengmaisan Formula Granule.关键词
生脉散/高效液相色谱法/人参皂苷Rg1/人参皂苷Re/同时测定Key words
Shengmaisan/HPLC/ginsenoside Rg1/ginsenoside Re/simultaneous determination分类
医药卫生引用本文复制引用
张加雄,王凌,苏永林,杨正中,潘雄..HPLC法同时测定生脉散配方颗粒中人参皂苷Rg1和Re的含量[J].西南国防医药,2017,27(9):916-918,3.基金项目
成都军区医学科学技术研究“十二五”项目计划(C12020) （C12020）
