摘要
Abstract
Objective To explore the effects and mechanism of berberine in the treatment of epilepsy in rats from oxidative stress and apoptosis.Methods Pilocarpine was used to establish rat epilepsy model.A berberine group (group B) was administrated with 25 or 50 mg/(kg·d) by gavage.Three days after modeling,the contents of multiple cytokines,enzyme,reactive oxygen species (ROS) and glutathione (GSH) in the hippocampus were inspected;10 days after modeling,the duration and frequency of epileptic episodes (≥ Level 4) in rats were observed by video recording;after 10 consecutive days of observation,NeuN dyeing was applied to determine the loss of neurons on the hippocampus.Results (1) the episode duration and frequency in group B-50 were significantly shorter or lower than those in group B-25(P ＜ 0.05),and the episode duration and frequency in the two groups were much shorter or lower than those in the epilepsy group (group S) (P ＜ 0.05).(2) Compared with group S,the ROS,HO-1,caspase3 and CTSD in the control group (group N) were significantly lower (P ＜ 0.05),but GSH,CAT and Nrf2 were much higher (P ＜ 0.05);the GSH,CAT and Nrf2 in group B-25 were significantly higher (P ＜ 0.05),but the caspase3 was much lower (P ＜ 0.05);the GSH,CAT,Nrf2 and HO-1 in group B-50 were significantly higher (P ＜ 0.05),but ROS,caspase3 and CTSD were much lower (P ＜ 0.05).(3) Compared with group B-25,the Nrf2 and HO-1 in group B-50 were significantly higher (P ＜ 0.05),but the caspase3 was much lower (P ＜ 0.05).(4)20 days after modeling,the number of neuron in area CA3 in group S was significantly lower (P ＜ 0.05);the number of neuron in the same area in group B-50 was higher than that in group B-25,but there was no significant difference(P ＞ 0.05).Conclusion Berberine can reduce the duration and frequency of epileptic seizures by inhibiting oxidative stress and apoptosis to reduce the loss of hippocampal neurons.关键词
癫痫/小檗碱/海马/神经元Key words
epilepsy/berberine/hippocampal neuron分类
医药卫生
