中国麻业科学2017,Vol.39Issue(4):189-194,6.
高效液相色谱法测定剑麻叶片中的可溶性糖含量
Determination of Soluble Sugar in Sisal Leaf by HPLC
摘要
Abstract
Different concentration ethanol extracting solutions were selected to study the extraction efficiency for glucose,fructose and sucrose.The high efficiency HPLC way of measuring the three soluble sugars simultaneously was expected to be constructed.The results showed that 60% ethanol was the optimal extraction solution,with relative standard deviations of 1.0% ~4.6%,association coefficients above 0.999 and the adding standard recoveries of 98.5% ~99.4%.The total soluble sugar contents were up to 167 mg/g,in which the glucose contents were the highest with the dry weight of 74 mg/g.关键词
高效液相色谱/剑麻/可溶性糖/叶片Key words
HPLC/sisal/soluble sugar/leaf分类
农业科技引用本文复制引用
鹿志伟,杨子平,张燕梅,李俊峰,侯晓婉,赵艳龙,陆军迎,周文钊..高效液相色谱法测定剑麻叶片中的可溶性糖含量[J].中国麻业科学,2017,39(4):189-194,6.基金项目
现代农业产业技术体系建设专项(CARS-19) (CARS-19)
湛江市热带作物遗传改良重点实验室建设项目(2015A06005) (2015A06005)
中国热带农业科学院南亚热带作物研究所基本科研业务费专项(1630062016019) (1630062016019)
国家自然科学基金(31401427) (31401427)