中国农业科技导报2017，Vol.19Issue(9)：24-33,10.DOI:10.13304/j.nykjdb.2016.684
结球甘蓝AUX/IAA家族基因BoIAA2与BoIAA19的克隆与表达分析
Cloning and Expression Analysis of BoIAA2 and BoIAA19 Genes of AUX/IAA Family in Cabbage
摘要
Abstract
To explore the function of AUX/IAA family genes on leaves and shoot tips development in cabbage,and screen the key AUX/IAA genes associated with leaves and shoot tips development,this study took cabbage line '519'as material and though transcriptome comparative analysis screened 2 AUX/IAA genes expressed significantly different in shoot and leaves tips during heading and rosette stages,named BoIAA2 and BoIAA19,respectively.The cDNA sequences of BoIAA2 and BoIAA19 gene were obtained by PCR.Their CDS length was 519 bp and 585 bp,and encoded 172,194 amino acids,respectively.Further sequences discovered that the comparability of BoIAA2 and BoIAA19 with BrIAA2 and BnIAA19 were 99％,which contained the typical AUX/IAA Domain Ⅰ,Ⅱ,Ⅲ and Ⅳ.The RT-PCR analysis showed that the relative expression levels of BoIAA2 and BoIAA19 genes during heading stage were higher than rosette stage both in leaves and shoot tips,which was completely consistent with expression trend of their binding protein genes BoTIR1 and BoARF8,suggesting that BoIAA2 and BoIAA19 might regulate auxin signal transduction by combining backward genes BoTIR1 with BoARF8,and participate in leaves and shoot tips development regulation in cabbage.关键词
结球甘蓝/AUX/IAA/基因克隆/表达分析Key words
Brassica oleracea L.var.capitata L./AUX/IAA/gene cloning/expression analysis分类
农业科技引用本文复制引用
蒲全明,施松梅,张林成,高启国,任雪松,向承勇,杨鹏,林帮民,耿明明..结球甘蓝AUX/IAA家族基因BoIAA2与BoIAA19的克隆与表达分析[J].中国农业科技导报,2017,19(9):24-33,10.基金项目
973计划项目(2012CB113900) （2012CB113900）
国家自然科学基金项目(30900986) （30900986）
现代农业产业技术体系四川蔬菜创新团队专项资助. （）
