中国动物传染病学报2017，Vol.25Issue(4)：34-39,6.
日本血吸虫mir-125b靶向调控宿主CD276的鉴定研究
IDENTIFICATION OF PATHOGEN mir-125b TARGETING MURINE CD276 IN MICE INFECTED WITH SCHISTOSOMA JAPONICUM
摘要
Abstract
To validate Schistosoma japonicum (S.japonicum) mir-125b targeting murine CD276 and analyze the expression of CD276 in different tissues of mice infected with S.japonicum,we predicted in our previous study that Schistosomajaponicum mir-125b might target murine CD276.Here,we used PCR to amplify the putatively interacted regions of murine CD276 and cloned the amplified fragment into the downstream of a Gaussia luciferase vector (pGluc-Basic).Then,the recombinant plasmids mir-125b mimics and anti-sense mir-125b were transfected into HEK293 T cells and the luciferase activities were measured by using a dual luciferase reporter system.In addition,the expression of CD276 in vitro cultured murine macrophages was also determined in quantitative real-time PCR upon mir-125b mimics transfection.Finally,the expression of CD276 in different tissues of mice infected with S.japonicum was also determined in quantitative real-time PCR.The putatively interacted region of CD276 was obtained and the corresponding recombinant plasmid was successfully constructed.A significant down-regulation of luciferase reporter activity was observed upon transfection ofmir-125b mimics into HEK293T cells (P ＜ 0.05).Transfection ofmir-125b mimics into murine macrophages led to the down-regulation of CD276.In addition,the expression of CD276 in lymphocytes,livers,and spleens of mice infected with S.japonicum was also down-regulated (P ＜ 0.05).Our results indicated that S.japonicum mir-125b directly target murine CD2 76 during schistosome infection,suggesting that mir-125b might play an important regulatory role in parasite-host interactions.关键词
日本血吸虫/mir-125b/CD276/功能Key words
S.japonicum/mir-125b/CD276/function分类
农业科技引用本文复制引用
王立辉,朱丽慧,刘军涛,夏天奇,陈永军,李建军,程国锋..日本血吸虫mir-125b靶向调控宿主CD276的鉴定研究[J].中国动物传染病学报,2017,25(4):34-39,6.基金项目
国家自然科学基金(31502056和31672550) （31502056和31672550）
中国农业科学院科技创新工程 （）
