中国动物传染病学报2017，Vol.25Issue(4)：64-67,4.
牛传染性鼻气管炎病毒gB基因荧光定量PCR检测方法建立
DEVELOPMENT OF TAQMAN PROBE REAL-TIME QUANTITATIVE PCR TO DETECT GB GENE OF BOVINE INFECTIOUS RHINOTRACHEITIS VIRUS
摘要
Abstract
In order to detect infectious Bovine rhinotracheitis virus (IBRV),a TaqMan fluorescence quantitative PCR detection method was developed using specifically paired primers and corresponding TaqMan probe targeting to the g,B gene of IBRV.After establishing and optimizing the reaction system,the sensitivity of the method was detected by a 10-fold dilution of the virus.At the same time,specific detection of Pseudorabies virus (PRV),Marek's disease virus(MDV) and Duck plague virus (DPV) was conducted.Results showed that the fluorescence PCR detection method based ongB gene can be used to identify IBRV,which has good sensitivity and specificity,and the sensitivity of 10 copies/u L,namely 0.02 TCID50,and no cross reaction with other virus.关键词
牛传染性鼻气管炎病毒/实时荧光定量PCRKey words
Bovine infectious rhinotracheitis virus/Real-time quantitative PCR分类
农业科技引用本文复制引用
王海军,王善辉,昌莉丽,陈鸿军..牛传染性鼻气管炎病毒gB基因荧光定量PCR检测方法建立[J].中国动物传染病学报,2017,25(4):64-67,4.基金项目
2014年徐州生物工程职业技术学院科研课题(2014B10) （2014B10）
