中国畜牧兽医2017，Vol.44Issue(5)：1267-1274,8.DOI:10.16431/j.cnki.1671-7236.2017.05.002
METTL3对乳腺上皮细胞中乳蛋白和乳脂肪合成的影响
Effect of METTL3 on Milk Protein and Milk Fat Synthesis of Bovine Mammary Epithelial Cells
摘要
Abstract
In this study,to investigate the regulatory role of methyltransferase-like protein 3 (METTL3)on milk protein and fat synthesis in bovine mammary epithelial cells (BMEC),differ-ent BMEC models were established,the expression of related genes and localization of METTL3 were detected,and the impact of METTL3 on milk protein and fat synthesis in BMEC after addingβ-estrogen (E),methionine (Met),and overexpression or inhibition of METTL3 were measured using Western blotting and imunofluorescience methods.The results showed that when E and Met were added or the METTL3 expression was promoted,the expressions of mTOR,p-mTOR, GlyRS,p-GlyRS,CSN2 and SREBP-1c were increased,oppositely,the gene expression were de-creased.Those results indicated that METTL3 could affect the milk fat and protein synthesis by adjusting the expression of related signaling pathway gene.METTL3 was one of the important regulating milk synthetic molecules and had an positively regulation on milk fat and protein syn-thesis in cells.关键词
m6A甲基转移酶/奶牛乳腺上皮细胞/乳脂肪/乳蛋白Key words
m6 A methyltransferase-like protein 3 (METTL3 )/bovine mammary epithelial cells (BMEC)/milk fat/milk protein分类
农业科技引用本文复制引用
李冬,黄鑫,李姗姗,于萌萌,李萌,张明辉,高学军..METTL3对乳腺上皮细胞中乳蛋白和乳脂肪合成的影响[J].中国畜牧兽医,2017,44(5):1267-1274,8.基金项目
国家自然科学基金项目(31472162) （31472162）
国家高技术研究发展计划(863计划)项目(2013AA102504-03) （863计划）
