中国畜牧兽医2017，Vol.44Issue(5)：1275-1280,6.DOI:10.16431/j.cnki.1671-7236.2017.05.003
米非司酮对小鼠精子穿透卵丘细胞层的影响
Effects of Mifepristone (RU486)on Sperm/Cumulus Penetration in Mice
摘要
Abstract
This study was aimed to investigate the effects of RU486 (mifepristone)on sperm pen-etration through the cumulus cells layer during fertilization in mice.After 20 μg/mL RU486 was added into the capacitation or the sperm/cumulus penetration medium,respectively,the experi-ments were conducted to evaluate the ratio of sperm acrosome reaction and ability of sperm/cu-mulus penetration.The results showed that the addition of RU486 significantly suppressed 5 μg/mL P4-induced acrosome reaction in the capacitated sperm (P <0.01),and decreased sperm pene-trating through the cumulus matrix and reaching the oocyte zona pellucid (ZP)and also remark-ably reduced the percentage of acrosome-reacted sperm within the oocyte-cumulus complex (OCC)(P <0.01).Compared with the addition of RU486 alone,P4 did not reverse the inhibitory effects of RU486 on the acrosome reaction of sperm within the OCC,though it still improved sperm penetration through the cumulus matrix and reaching the ZP (P < 0.01 ).Therefore, RU486 could inhibit P4-induced acrosome reaction and decrease sperm penetration through the cu-mulus matrix,which suggested that P4/progesteron receptor (PGR)pathway might be very im-portant for sperm penetration through the cumulus cell layer.关键词
米非司酮/孕酮/精子-卵丘互作/顶体反应/精子Key words
RU486/P4/sperm-cumulus interaction/acrosome reaction/sperm分类
农业科技引用本文复制引用
周崇,黄莉,石德顺,蒋建荣..米非司酮对小鼠精子穿透卵丘细胞层的影响[J].中国畜牧兽医,2017,44(5):1275-1280,6.基金项目
广西自然科学基金(2014GXNSFCA118008) （2014GXNSFCA118008）
广西博士后基金(Y334002001) （Y334002001）
